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Volume 9
Journal of Analytical & Bioanalytical Techniques
Mass Spectra & Analytika 2018
September 19-20, 2018
September 19-20, 2018 Singapore
World Congress on
Mass Spectrometry and Analytical Techniques
Maria Ramos Payan et al., J Anal Bioanal Tech 2018, Volume 9
DOI: 10.4172/2155-9872-C2-031
Development, optimization and validation of a method for the determination of veterinary drugs from
different therapeutic classes in animal urine by high resolution liquid chromatography
Maria Ramos Payan, García González, Juan Antonio Ocaña, Rut Fernández and Miguel Ángel Bello
University of Seville, Spain
A
short-time, precise and robust HPLC separation procedure has been developed and optimized for the determination of a
series of drugs of different therapeutic classes: chlortetracycline, oxitetracycline, cefoperazone, diclofenac, tiamphenicol,
marbofloxacin, ciprofloxacin, danofloxacin, enrofloxacin and flumequine. The chromatographic method used a monolithic C
18
column and both diode array and fluorescence detection. This procedure was validated for the analysis of drugs in cow urine,
using a simple and fast extraction procedure with methanol/acetonitrile, allowing the simultaneous and efficient extraction
of most of the studied drugs. The proposed method was successfully applied to the determination of enrofloxacin (and its
metabolite ciprofloxacin) in cow urine, collected after the administration of this antibiotic. A robust and simple HPLC method
has been developed to the separation of 11 veterinary drugs of different therapeutic classes. The employed monolithic C
18
column (with high porosity and permeability) allowed the application of high volume flows with resulting tolerable precision
flow levels and achieved an efficient separation within a short time (12 minutes). The DAD and FLD systems allowed the
quantification of the studied drugs with low LOD and LQD and good repeatability. Also, a simple and fast extraction procedure
has been developed and applied to the determination of drugs in cow urine. The procedure allowed the extraction of most of
the studied drugs, achieving high recoveries with low sample volume consumption. The procedure was applied to the analysis
of cow urine after an enrofloxacin treatment allowing its quantification.
Biography
Maria Ramos Payan has pursued her PhD from University of Seville, Spain and Postdoctoral studies from University of Copenhagen, Denmark, University of North
Carolina, USA and Microelectronic National Center of Barcelona, Spain. She is the Leader of the microfluidic research line. She has published more than 30 papers
in reputed journals and has been serving as an Editorial Board Member of repute.
ramospayan@us.es