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Volume 3 Diagnostic Pathology: Open Access
ISSN: 2476-2024 Laboratory Medicine 2018
June 25-26, 2018
June 25-26, 2018 | Berlin, Germany
13th International Conference on
Laboratory Medicine & Pathology
Thymoquinone synergizes the anticancer properties of cisplatin against head and neck squamous cell
carcinoma and protects normal oral epithelial cells
Omar M Al Aufi1, Abdulwahab Noorwali2, Fatheya Zahran3, Ahmed M Al Abd2,4,5, Safia Al Attas 2 and Fahd M Almtutairi1
1Comperhensive Specialized Ployclinic for Security Forces in Medina, Saudi Arabia.
2King Abdulaziz University, Saudi Arabia
3Cairo University, Egypt
4National Research Centre, Egypt
5Nawah Scientific, Egypt
C isplatin (CDDP) is a potent anticancer agent used for several tumor types. Thymoquinone (TQ) is naturally occurring
compound drawing great attention as anticancer and chemomodulator for chemotherapies. Herein, we studied the
potential cytotoxicity of thymoquinone, CDDP and their combination against human oral squamous cell carcinoma cell in
contrast to normal oral epithelial cells. CDDP similarly killed both head and neck squamous cell carcinoma cells (UMSCC-
14C) and normal oral epithelial cells (OEC). TQ alone exerted considerable cytotoxicity against UMSCC-14C cells; while
it induced weaker killing efect against normal oral epithelial cells (OEC). Equitoxic combination of TQ and CDDP showed
additive to synergistic interaction against both UMSCC-14C and OEC cells. TQ alone increased apoptotic cell fraction in
UMSCC-14C cells, as early as after 6 hours. In addition, prolonged exposure of UMSCC-14C to TQ alone resulted in 96.7±1.6%
total apoptosis which was increased after combination with CDDP to 99.3±1.2% in UMSCC-14C cells. On the other hand,
TQ induced marginal increase in the apoptosis in OEC and even decreased the apoptosis induced by CDDP alone. Finally,
apoptosis induction results were confirmed by the change in the expression levels of p53, Bcl-2 and Caspase-9 proteins in both
UMSCC-14c and OEC cells.
Omar M Al Aufi et al., Diagn Pathol Open 2018, Volume 3
DOI: 10.4172/2476-2024-C1-002