14 / 14
Page 59
Notes:
conferenceseries
.com
Volume 3
Diagnostic Pathology: Open Access
ISSN: 2476-2024
Laboratory Medicine 2018
June 25-26, 2018
June 25-26, 2018 | Berlin, Germany
13
th
International Conference on
Laboratory Medicine & Pathology
In vivo
CFTR function by imaged ratiometric measurement of beta adrenergic/cholinergic sweat rate
in human sweat glands
Paola Melotti
Centro Fibrosi Cistica-Azienda Ospedaliera Universitaria Integrata Verona piazzale Stefani, Italy
S
weat secretion rates were given by changes of volume of sweat drops secreted on the forearm in an oil layer, including the
presence of a water-soluble blue dye (erioglaucine disodium crystals). We computed a ratio between CFTR-dependent,
evoked by intradermal microinjection of a β-adrenergic cocktail (C-sweat), and CFTR-independent, induced by methacoline
as cholinergic stimulus (M-sweat), sweat secretion rates by multiple individual glands. The analysis was performed in 22
CF patients, 22 non-CF subjects (CTR), 22 healthy carriers (HTZ) and 3 clinical cases. Sweat secretion rates were given by
changes of volume of sweat drops secreted on the forearm in an oil layer, including the presence of a water-soluble blue dye
(erioglaucine disodium crystals). We computed a ratio between CFTR-dependent, evoked by intradermal microinjection of
a β-adrenergic cocktail (C-sweat), and CFTR-independent, induced by methacoline as cholinergic stimulus (M-sweat), sweat
secretion rates by multiple individual glands. The analysis was performed in 22 CF patients, 22 non-CF subjects (CTR), 22
healthy carriers (HTZ) and 3 clinical cases. We obtained an approximately linear readout of CFTR function: the carriers mean
ratio was 0.51 the value of non-CF controls while the average ratio of CF subjects was around zero. In a patient affected by CFTR
related disorder we found a value in between CF and HTZ mean values. All groups were clearly discriminated with extremely
significant differences of C-sweat/M-sweat ratios (p<0.0001 for three groups comparison). This method discriminates between
CF and non-CF patients (non-CF controls and heterozygotes), providing sensibility and specificity of 100%. It discriminates
between heterozygotes and non-CF controls, providing sensibility 82% of specificity of 86%. We obtained reproducible
discrimination when different operators performed the test. A software was developed for detecting sweat bubbles, paving
the way for automatically mapping and measuring sweat bubbles as required for automated image analysis. This bioassay is
capable to clearly discriminate among non CF, healthy carriers and CF individuals at variance with Gibson and Cooke gold
standard sweat chloride assay, is minimally invasive and thanks to its exquisite sensitivity and specificity appears suitabile for
multicentre studies focusing on CFTR targeted therapies and to assist in the diagnosis of controversial cases. This approach can
simplify the analysis and thus promote a better understanding of the functional relevance of rare CFTR mutations.
Biography
Paola Melotti is currently working Cystic Fibrosis Center of Verona, Italy. Paola Melotti has done progressive work on "
In vivo
CFTR function by imaged ratiometric
measurement of beta adrenergic/cholinergic sweat rate in human sweat glands" at University of Verona,Department of Pathology and Diagnostics.
paola.melotti@aovr.veneto.itPaola Melotti, Diagn Pathol Open 2018, Volume 3
DOI: 10.4172/2476-2024-C1-003