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Volume 7 Journal of Infectious Diseases & Therapy Infectious Diseases & Endocrinology 2019
February 27-28, 2019
February 27-28, 2019 Tokyo, Japan
Infectious Diseases, Diabetes and Endocrinology
Global Experts Meeting on
Maham S et al., J Infect Dis Ther 2019, Volume 7
DOI: 10.4172/2332-0877-C2-062
BACTEC and conventional culture systems comparison to isolate of microorganisms from blood
and other sterile body fluids
Maham S1, Shirvani F1, Hadipour Jahromi M1, Fallah F1, Razavi S S1, Nikfarjam A2 and Rahbar M2
1Shahid Beheshti University of Medical Sciences, Iran
2Ministry of Health and Medical Education, Iran
Blood stream infectious diseases are prevalent in children and adults. Rapid and accurate diagnosis of bloodstream infections
are primarily based on conventional culture results, which saves time and prevents empiric treatment. This study aims to
BACTEC and conventional culture systems comparison to isolate of microorganisms from blood and other sterile body fluids,
on blood specimens collected from three training hospitals in Tehran, Iran. BACTEC 9120 and conventional methods were
used for isolation of microorganism from specimens including blood and other body fluid samples collected from patients
hospitalized in the selected hospitals during the study period, from April to June 2009. Time for positive and negative results
and hospital charge were estimated for the two culture methods. In total 747 specimens were possessed by BACTEC 9120
systems and 787 by conventional method. Patients aged between 3 days and 8 years old, (mean 11.4±21.9 years); 52% of patients
were male and 48% female; Out of 747 specimens were possessed by BACTEC 9120 system, 26% (196/747) and from 787
specimens cultured by conventional method 5% (49/787) were positive (p<0.05). Hospital stay was 13.8±12.9 days in BACTEC
9120 and 17.9±14.9 days in Conventional method (p<0.05), respectively. Time for positivity by BACTEC system was 3.8±1.1
days and 5.9±2.5 days in conventional method (p<0.05), treatment response showed 1.8 day earlier result in patients that their
specimens were processed by BACTEC 9120 system. Death rate in BACTEC 9120 method was 6% and 11% in conventional
method (p>0.05). In conclusion implementation of BACTEC system for microbiologic detection of pathogens decreases the
admission time and early diagnosis and treatment results are cost effective for patient management and prevention of antibiotic
resistance.
Biography
Maham S is working as the Manager of Pediatric Infections Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. His research interest
is in medical and diagnostic methods.