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conferenceseries
.com
Volume 7
Journal of Infectious Diseases & Therapy
Infectious Diseases & Endocrinology 2019
February 27-28, 2019
February 27-28, 2019 Tokyo, Japan
Infectious Diseases, Diabetes and Endocrinology
Global Experts Meeting on
J Infect Dis Ther 2019, Volume 7
DOI: 10.4172/2332-0877-C2-063
Genetic diversity of Hepatitis C virus in Pakistan using next generation sequencing
Sana Saleem
University of the Punjab, Pakistan
I
n Pakistan, HCVdisease is considered amajor public health issue with about 10-17million people suffering with this infection
and rate is increasing every day without any hindrance. The currently available pyrosequencing approach is used to analyze
complex viral genomes as it can determine minor variants. It is crucial to understand viral evolution and quasispecies diversity
in complex viral strains. Intra-host viral diversity of HCV was determined using Next Generation Sequencing (NGS) from 13
chronically HCV infected individuals. NGS of three different regions (E2 (HVR1), NS3 and NS5B) of HCV-3a allowed for a
comprehensive analysis of the viral population. Phylogenetic analysis of different HCV genes revealed great variability within
the Pakistani population. The average nucleotide diversity for HVR1, NS3 and NS5B was 0.029, 0.011 and 0.010, respectively.
Our findings clearly indicate that patient-2 had greater quasispecies heterogeneity than other patients of same genotype-3a
using phylogenetic and one step network analyses. Initially phylogenetic analysis showed that genotype 3a samples have greater
genetic diversity. However, no significant difference was determined when nucleotide variability of genotype 3a compared with
other genotypes (1a, 1b, 2a and 4a).
sanacemb@yahoo.com