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.com
Volume 5, Issue 7 (Suppl)
J Infect Dis Ther, an open access journal
ISSN: 2332-0877
Infection Prevention 2017
December 14-15, 2017
December 14-15, 2017 | Rome, Italy
13
th
World Congress on
INFECTION PREVENTION AND CONTROL
Fecal carriage of carbapenem resistance
Enterobacteriaceae
among inpatients in a university hospital
in Iran
Fereshteh Shahcheraghi
Pasteur Institute of Iran, Iran
Objectives:
Fecal colonization by carbapenem-resistant
Enterobacteriaceae
(CRE) could serve as a reservoir for transmission
of these pathogens to clinical settings, which subsequently increases clinical infections. The aim of this study was to evaluate
the prevalence and risk factors associated with CRE fecal colonization among inpatients.
Material &Methods:
Rectal swabs from 50 patients in a university hospital were collected. CRE screening was performed
by using selective media. Carbapenemase production was detected by phenotypic tests. PCR assays were used to detect
carbapenemases genes. Clonal relatedness was investigated by pulsed-field gel electrophoresis (PFGE).
Results:
The prevalence of fecal colonization was 56% (28/50). Overall, 41 CRE isolates were identified, of which 38 were
carbapenemase-producers. Eleven patients (39.3%) were co-colonized with CRE isolates. ICU hospitalization, prior antibiotic
therapy, and mechanical ventilation were significant risk factors. The
blaOXA-48
was the most frequent carbapenemases
followed by
blaNDM-1 and blaNDM-7
enzyme. Nine carpapenemase producing
Enterobacteriaceae
(CPE) isolates co-harbored
blaNDM-1 and
blaOXA-48
. Also, six CPE isolates co-harboredblaNDM-7 and
blaOXA-48
.We did not detect
blaKPC, blaGES,
blaIMP and blaVIM
. PFGE analysis showed that
E. coli
clones were diverse, while
K. pneumoniae
categorize in 3 clusters.
Cluster I was the major clone carrying
blaOXA-48and blaCTXM-15
genes.
Conclusions:
Our study as the first investigation in Iran showed CRE not only had high prevalence in fecal carriages, but also
harbored varied antimicrobial resistance elements.
Biography
Fereshteh Shahcheraghi is the Head of the Bacteriology Department of Pasteur Institute of Iran. She obtained her PhD in Medical Microbiology in 1996 and
joined the Institute Pasteur in 1997 as Assistant Professor. From 2002-2003, she went to Japan for studying and researching on antibiotic resistance. Her main
field is antibiotic resistance especially on Gram Negative Bacteria. She has several projects and papers on CRE (carbapenem Resistance
Enterobacteriaceae
) in
outpatients and in patients in Iran. She is the Head of Pertussis National Reference Lab of Pasteur Institute; this lab has collaboration with CDC of Iran for diagnosis
of suspected patients to Pertussis and research on isolated strains. Also she has international project on Pertussis. She has authored more than 60 articles in
international peer-reviewed journals and several national and international projects she is also actively involved in research, directing studies of post-graduate
students, post-doctoral research workers and trainees.
shahcheraghifereshteh@yahoo.comFereshteh Shahcheraghi, J Infect Dis Ther 2017, 5:7(Suppl)
DOI: 10.4172/2332-0877-C1-035