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Journal of Infectious Diseases & Therapy |ISSN: 2332-0877 | Volume 6
June 25-26, 2018 | Vancouver, Canada
3
rd
International Conference on
2
nd
International Conference on
Infection, Disease Control and Prevention
Microbial Pathogenesis & Infectious Diseases
&
Association of
Helicobacter pylori
biofilm with enterovirus 71 prolongs viral viability and survival
Vincent TK Chow
1
, A M Hassanbhai
2
, M C Phoon
1
, B Ho
1
1
National University of Singapore, Kent Ridge, Singapore
2
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore
V
iruses are unable to replicate outside their hosts, and thus the transition time during which a virus leaves its host and
infects the next susceptible host is critical for virus survival. Closely associated with hand, foot and mouth disease with
occasional neurologic complications, enterovirus 71 (EV71) is stable in aqueous environments. However, its survival in the
environment and interactions with bacteria are not well-established. On the other hand,
Helicobacter pylori
is a well-known
and highly successful gut bacterial pathogen that infects 50% of individuals, with its capacity to form biofilms being linked
with its transmission. We hypothesized that bacterial biofilm may play a significant role in the survival of EV71 in the external
environment. In this study, we examined the interactions of EV71 with the biofilm of
H. pylori
. The results reveal that EV71
associated with
H. pylori
biofilm as observed under confocal and scanning electron microscopy. Furthermore, the presence of
biofilm prolonged viral viability as demonstrated by virus plaque assays. Interestingly, the viability of the virus was dependent
on the quantity of
H. pylori
biofilm formation. Taken together, the ability of bacterial biofilm in extending EV71 viability for
prolonged periods may partially contribute to EV71 outbreaks, and implies that the association of the virus with bacterial
biofilm may serve as a potential pathway of EV71 transmission.
Biography
Vincent TK Chow, MD, PhD, FRCPath completed his postgraduate studies at the National University of Singapore (NUS) and the University of London, UK. He is
an Associate Professor of Microbiology and Principal Investigator of the Host And Pathogen Interactivity Laboratory, NUS. Dr Chow has published more than 250
papers in international journals, and has been awarded several research prizes and patents.
micctk@nus.edu.sgVincent TK Chow et al., J Infect Dis Ther 2018, Volume 6
DOI: 10.4172/2332-0877-C2-041