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Volume 6 Journal of Infectious Diseases and Therapy
ISSN: 2332-0877 Infection Congress 2018
March 01-02, 2018
March 01-02, 2018 Berlin, Germany
5th International Congress on
INFECTIOUS DISEASES
Quantitative analysis of Human herpes virus 6 DNA in patients treated for acute leukemia
Radwa Hussein Mohamed Ghoraba, Ghada F Helaly, Nadia E Zaki and Dalia E Metwally
Alexandria University, Egypt
Viral infections are important causes of morbidity and mortality for patients with a hematological malignancy, but the
true incidence and consequences of viral infections for these patients who undergo conventional non transplant therapy
are inadequately defined. The significance of estimating the prevalence of HHV-6 in cancer patients does not actually get
its importance from morbidity of the disease itself but from further interference of the outcome in bone marrow transplant
patients; where it may be associated with serious life threatening complications. Thus, screening of patients with hematological
malignancies for HHV-6 might be considered mandatory. The aim of this study was to evaluate a possible association between
HHV-6 infection and acute leukemia in adults after receiving chemotherapy treatment for acute leukemia. The patients were
divided into two main groups according to the type of leukemia: Group I; 36 patients with newly diagnosed acute myeloid
leukemia (AML) and group II; 27 patients with newly diagnosed acute lymphoblastic leukemia (ALL); 21 patients with B-ALL
and six patients with T-ALL. All 63 studied adult patients with newly diagnosed acute leukemia were subjected to history
taking, complete clinical examination for the presence of organomegaly and routine laboratory investigations. Peripheral
blood samples were collected from all patients for quantitative determination of HHV6 viral load by Taqman probe technique
(real time PCR) at day 0 and day 100 of induction chemotherapy. The results argued against an etiological relationship between
HHV-6 infection and the genesis of acute leukemia in adults, however, it supports the hypothesis of viral latency and the
possibility of virus reactivation in immunocompromised hosts. The possible presence of HHV-6 as an associated or a putative
causative agent in leukemia should however be considered. Recommendations include screening of patients with hematological
malignancies for HHV-6 might be considered among the routine initial laboratory work-up.
Recent Publications
1. Agut H, Bonnafous P and Gautheret-Dejean A (2015) Laboratory and clinical aspects of human herpesvirus 6
infections. Clin Microbiol Rev 28(2):313-35.
2. Pellett P E, Ablashi D V, Ambros P F, Agut H, Caserta M T, Descamps V, et al. (2012) Chromosomally integrated
human herpesvirus 6: questions and answers. Rev Med Virol 22(3):144-55.
3. Becerra A, Gibson L, Stern L J and Calvo-Calle J M (2014) Immune response to HHV-6 and implications for
immunotherapy. Curr Opin Virol 9:154-61.
4. Yip C C Y, Sridhar S, Cheng A K W, Fung A M Y, Cheng V C C, Chan K H, et al. (2017) Comparative evaluation of
a laboratory developed real-time PCR assay and the RealStar® HHV-6 PCR Kit for quantitative detection of human
herpesvirus 6. J Virol Methods 2017; 246:112-6.
5. Inazawa N, Hori T, Yamamoto M, Hatakeyama N, Yoto Y, Nojima M, et al. (2016) HHV-6 encephalitis may complicate
the early phase after allogeneic hematopoietic stem cell transplantation: Detection by qualitative multiplex PCR and
subsequent quantitative real-time PCR. J Med Virol. 88(2):319-23.
Biography
Radwa Hussein Mohamed Ghoraba is a Pharmacist, graduated from Faculty of Pharmacy and Drug Manufacturing, Pharos University, Alexandria, Egypt in 2012.
She completed her Master’s degree in Diagnostic and Molecular Microbiology at Medical Research Institute (MRI), Alexandria University in 2017. Her involvement
in research has given her first-hand exposure to the process of active scientific research, resulted in incredible research experiences, and instilled in her a passion
for science and exploration. She is interested in improving public health through research.
Radwa Hussein Mohamed Ghoraba et al., J Infect Dis Ther 2018, Volume 6
DOI: 10.4172/2332-0877-C1-039