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.com
Volume 5, Issue 2 (Suppl)
J Infect Dis Ther 2017
ISSN: 2332-0877, JIDT an open access journal
Infection Congress 2017
May 11-12, 2017
May 11-12, 2017 Barcelona, Spain
4
th
International Congress on
Infectious Diseases
Dalia Salem et al., J Infect Dis Ther 2017, 5:2 (Suppl)
http://dx.doi.org/10.4172/2332-0877-C1-024Detection of
Helicobacter pylori
vacA, cagA
and
iceA1
virulence genes associated with gastric diseases in
Egyptian patients
Dalia Salem
1
, Ahmed El-Shenawy
1
, Manal Diab
1
, Mohamed Shemis
1
, Maged El-Ghannam
1
, Moustafa Abdelnasser
2
, Mohamed Shahin
1
, Mahmoud Abdel-
Hady
2
, Effat El-Sherbini
1
and
Mohamed Saber
1
1
Theodor Bilharz Research Institute, Egypt
2
Al-Azhar University, Egypt
H
elicobactor pylori (
H. pylori
) virulence markers would be useful to predict peptic ulcer disease (PUD) or gastric cancer. In
Egypt, since inadequate data are present regarding
H. pylori
virulence–related genes in different age group patients with gastro-
duodenal diseases, it becomes crucial to study the clinical status of
cagA, vacA
and
iceA1
genotypes of
H. pylori
strains recovered
from patients with dyspepsia. The study included 113 dyspeptic patients who were exposed to upper gastrointestinal endoscopic
examination. Four antral biopsies were obtained from each patient for the analysis of
H. pylori
infection by rapid urease test and
detection of 16S rRNA. 60 (53.1%) patient were confirmed to be infected with
H. pylori
. Upon endoscopy gastritis was revealed in 27
(45%) and 10 (16.7%) had PUD. Of the 60
H. pylori
strains, 39 (65%) had at least one virulence gene. Six different genotypic forms
were recognized; vacA (9/60),
iceA1
(1/60), vacA/cagA (7/60), vacA/
iceA1
(13/60), vacA/cagA/
(8/60), only one cagA/iceA
type and we could not detect cagA. The overall vacA, iceA1 and cagA genes were identified (61.6%, 38.8%, 26.6% respectively) by
PCR-based molecular testing. The vacA gene status was highly significant related to gastritis patient (P≤0.036). The vacA s1m1 and
s2m2 alleles were significantly found in 50% of
H. pylori
infected patients with PUD and with gastritis 57.1% respectively (P≤0.01).
In conclusion, the main genotype combinations in the studied Egyptian patients were; vacAs2m2/
iceA1
, vacAs1m1/cagA, mostly
associated with gastritis, and
vacAs1/cagA/icA
, mainly in PUD. The less virulent (s2, s2m2)
H. pylori
genotypes were found in patients
aged over 43 years.
Biography
Dalia Salem is a Lecturer of Medical Microbiology at Theodor Bilharz Research Institute- Egypt. Her special research interest and expertise is in “Detection of
emerging mechanisms of multi-drug-resistant bacteria and their susceptibility patterns to novel antibiotics in addition to antimicrobial resistant genes and virulence
markers in
Helicobacter pylori
-related infections”.
drdaliasalem@gmail.com