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Industrial Chemistry | ISSN: 2469-9764 | Volume 4
17
th
International Conference on
May 21-22, 2018 | New York, USA
Industrial Chemistry and Water Treatment
Identification of genotoxicity in bioindicators produced by wastewater used for agricultural
irrigation
Perez-Nava Jessica
1
, Hernandez-Aldana F
1
, Rivera-Tapia A
1
, Martinez-Valenzuela C
2
, Tamariz-Flores V
1
and
Huerta-Lara M
1
1
Benemerita Autonomous University of Puebla, Mexico
2
Universidad Autónoma de Occidente, Mexico
P
opulation growth, industrial development, intensified agricultural production and the number of contaminants
incorporated into the environment have deteriorated the water compartments, it is necessary to develop strategies to
reduce and prevent their contamination. In some types of wastewater, products resulting from the biological treatment process
(non-biodegradable) may have significant toxicity and high genotoxicity. Genotoxicological effects may manifest at different
levels, from subcellular structures or enzyme systems, to whole organisms. The unicellular electrophoresis/Comet assay (EC)
and micronucleus are sensitive methodologies, available, cheap and applicable to any cell type to measure DNA strand breaks
in individual cells considered as assays indicative of premutagenic lesions. The evaluation of abnormal cells was performed in
producers using treated wastewater for agricultural irrigation. The presence of nuclear and micronuclei abnormalities in the
identified cells was classified in relation to their nuclear form by observing degenerative nuclear changes related to cellular
toxicity. Among the nuclear abnormalities detected in the producers prevail the condensed and binucleated cell nuclei, without
observation of more than six micronuclei per thousand cells counted; however, tests on bioindicators (
Allium sativum
and
Vicia faba
) will be carried out to confirm genotoxicity levels generated by the compounds present in the wastewater.
Biography
Perez-Nava Jessica has completed her MSc from Benémerita Universidad Autónoma de Puebla. Currently, she is pursuing her Doctoral program in the same
university. Her research areas include: photochemical treatment of wastewater, genotoxicity and microbiological tests. She has published two scientific papers and
has made academics mobilities in Mexico and abroad.
jesyolpris_1@hotmail.comPerez-Nava Jessica et al., Ind Chem 2018, Volume 4
DOI: 10.4172/2469-9764-C1-009