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conferenceseries
.com
Volume 3, Issue 2 (Suppl)
Ind Chem
ISSN: 2469-9764, ICO an open access journa
Industrial Chemistry 2017
May 22-23, 2017
May 22-23, 2017 Las Vegas, USA
2
nd
World Conference on
Industrial Chemistry and Water Treatment
Inactivation and degradation of antibiotic-resistant bacteria and its gene by Cu (II)/H
2
O
2
system
Byung-Taek Oh, Young-Seok Seo, Seol Ah Kim, Jung-Muk Lim, Jeong Yeol Lee, Aerin Kim, Gyunggeun Lee, Sewon Lee
and
Min Cho
Chonbuk National University, South Korea
T
his study deals with the degradation and/or removal of antibiotic resistant bacteria (ARB) and antibiotic resistant gene
(ARG) using Cu(II)/H
2
O
2
. The removal of ARB using Cu(II) was achieved, however, the inactivation capability was
considerably improved when supplement with H
2
O
2
. The improved ARB inactivation was confirm by: (1) Addition of copper
chelating agents, EDTA (for Cu(II)) and DMP (for Cu(I)) to block Cu(II)/Cu(I) redox cycle, (2) Addition of radical scavenger
t-BuOH proving that ARB is mainly inactivated by Cu(III), and (3) Addition of H
2
O
2
to produce Cu(I) and Cu(III). To
investigate the cell destruction, PI staining was applied to check cell membrane integrity, and cell-permeability test to identify
intra- and extra-cellular oxidative damage. In case of ARG inactivation, the efficiency was up to 5.5% when Cu(II) was treated
alone, then it was improved up to 85% within 20 min when supplement with H
2
O
2
in the reaction. Therefore, it is concluding
that Cu (II)/H
2
O
2
system is not only potential for inactivation of ARB but also inactivation of ARG under neutral pH condition.
Biography
Byung-Taek Oh is an Environmental Scientist, Author and Educator, who developed a new strategy in toxic pollutant remediation field. His expertise and contextual
evaluation model based on responsive constructivists creates a new pathway for improving environmental pollution removal. He has framed a network in research
to create a model to remediate microbes and toxic pollutant from environment has added a feather to his cap. He has built this model after years of experience
in research, evaluation, teaching and administration both in laboratory and education institutions. The research foundation is based on his American carrier with
network development and his excellence in environmental research by measurement, description and judgment. He has awarded with excellence in several
aspects of research by his university and in several research occasions.
btoh@jbnu.ac.krByung-Taek Oh et al., Ind Chem 2017, 3:2 (Suppl)
http://dx.doi.org/10.4172/2469-9764-C1-006