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Volume 8, Issue 10 (Suppl)
J Diabetes Metab
ISSN: 2155-6156 JDM, an open access journal
Herbal Diabetes 2017
November 02-04, 2017
November 02-04, 2017 Bangkok, Thailand
23
rd
International Conference on
Herbal and Alternative Remedies for
Diabetes and Endocrine Disorders
Kinetic of α-amylase inhibition by
Salvia mirzayanii
extract and its fractions, as a model for treatment diabetes
Soheila Moein
1
, Mahmood Reza Moein
2
, Elham Pimoradloo
3
and Mahmood Vessal
4
1
Hormozgan University of Medical Sciences, Iran
2
Shiraz University of Medical Sciences, Iran
3
Islamic Azad University, Iran
4
Islamic Azad University of Shiraz, Iran
Introduction & Aim:
Type-2 Diabetes Mellitus (T2DM) affects a large population worldwide. This disease is a complex
heterogeneous group of metabolic disorders including hyperglycemia and impaired insulin action and/or insulin secretion.
Amylase inhibitors can reduce carbohydrate digestion rate and have the potential to prevent development of type-2 diabetes
mellitus. The Labiatae is one of the largest plant families grown globally and one of the members of this family is
Salvia
mirzayanii
Rech. F. & Esfand, which used for treatment diabetes. In present study, inhibitory potential of
Salvia mirzayanii
extract and its different fractions were investigated.
Methodology & Theoretical Orientation:
Ethanol extract of this plant leaves was fractionated using petroleum ether,
chloroform, ethyl acetate and n-butanol solutions. To measure enzyme inhibition enzyme, substrate and samples were mixed
and for evaluation mode of inhibition, constant amounts of α-amylase were incubated with increasing concentrations of
substrate. Acarbose was used as a standard.
Results:
The results showed that ethanol extract of
Salvia mirzayanii
was the most potent inhibitors of α-amylase in comparison
with the other samples. All the samples exhibited uncompetitive inhibition except Petroleum ether fraction which showed
competitive inhibition.
Conclusion & Significance:
Ethanol extract of
Salvia mirzayanii
showed the highest α-amylase inhibitory activity. In this
study, most of the samples are uncompetitive inhibitors and one of the advantages of these inhibitors is that they would be
effective at lower concentrations of substrate in comparison with competitive inhibitors which require higher concentrations
of the substrate for the same effects.
soheila_9@yahoo.comSoheila Moein et al., J Diabetes Metab 2017, 8:10 (Suppl)
DOI: 10.4172/2155-6156-C1-073