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Volume 8, Issue 10 (Suppl)
J Diabetes Metab
ISSN: 2155-6156 JDM, an open access journal
Herbal Diabetes 2017
November 02-04, 2017
November 02-04, 2017 Bangkok, Thailand
23
rd
International Conference on
Herbal and Alternative Remedies for
Diabetes and Endocrine Disorders
Phytosterols isolated from
Aloe barbadensis Mill
., restore reproductive and metabolic complications in
Letrozole-induced PCOS mouse model
Arpi Dey, Radha Maharjan and Laxmipriya Nampoothiri
Maharaja Sayajirao University of Baroda, India
Statement of the Problem:
Polycystic Ovary Syndrome (PCOS) is a multifactorial reproductive disorder which affects 4-12%
of women and is a leading cause of female infertility worldwide. PCOS is related to dyslipidemia, hyperandrogenism and
hyperinsulinemia. Treatment of these dysfunctions is by administration of steroid analogues and insulin sensitizers. However,
the use of these synthetic drugs gives rise to severe side effects. Hence, there is an immediate pre-requisite of an alternative
herbal therapy. The purpose of this study is to understand the role of phytosterols isolated fromAloe barbadensis Mill., towards
management of PCOS.
Methodology &Theoretical Orientation:
Aloe vera gel was extracted used petroleum ether and the extract thus obtained was
fractionated using silica gel column chromatography. Phytochemical screening, thin layer chromatograph, HPTLC and GC/
MS were done to characterize the obtained phytosterols. Letrozole (0.5 mg/kg body weight) induced PCOS mouse model was
fed orally with the isolated phytosterol for 60 days. After completion of the treatment, several metabolic as well as reproductive
parameters were evaluated.
Findings:
Detailed phytochemical screening demonstrated the presence of several phytosterols such as beta-sitosterol,
stigmasterol and campesterol. Upon treatment with the isolated phytosterols, letrozole induced PCOS mouse model showed
an improvement in the glucose sensitivity, restored lipid profile and estrus cyclicity, decreased the serum testosterone levels and
increased the serum progesterone and estradiol levels. The animals also exhibited disappearance of the peripheral cysts, which
is a hallmark of PCOS. The phytosterols elicit their effectiveness by getting bio-transformed into oxysterols, which can further
influence molecular pathways that mainly regulate steroidogenesis and lipid metabolism.
Conclusion & Significance:
This study is first of its kind which demonstrates the direct effect of phytosterols isolated from
Aloe vera gel at molecular level towards management of PCOS, thereby, adding to its overall potential and economic viability
at national and international level.
Biography
Arpi Dey is currently a PhD student at the Department of Biochemistry, The Maharaja Sayajirao University of Baroda, India. She has been extensively trained in the
domain of reproductive endocrinology. She has contributed greatly to the development of novel herbal therapeutics by exploring the knowledge of Indian heritage
of Ayurveda. She has expertise in animal handling, phytochemistry, molecular biology and cell-culture techniques.
arpi08.dey@gmail.comArpi Dey et al., J Diabetes Metab 2017, 8:10 (Suppl)
DOI: 10.4172/2155-6156-C1-073