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conferenceseries
.com
Volume 8
Journal of Gastrointestinal & Digestive System
GI Diseases 2018
July 11-12, 2018
July 11-12, 2018 Sydney, Australia
15
th
International Conference on
Digestive Disorders and Gastroenterology
Preconditioning and hypothermia as a protective effect for liver reperfusion injury due to femoral
artery ligation on
Oryctolagus cuniculus
Sinta Chaira Maulanisa
1
and Yefta Moenadjat
2
1
University of Indonesia, Indonesia
2
Dr. Cipto Mangunkusumo National Central General Hospital, Indonesia
Introduction:
Ischemia-reperfusion injury (IRI) causes damage to cells that are remote from the ischemic organ. Protective
strategies have been developed for protection of organs from ischemia reperfusion injury, which are ischemic pre-conditioning
(IPC) and hyportemia. IPC has been proven to decrease tissue injuries through resistance mechanisms towards ischemia
and lower energy requirements. Meanwhile, hypothermia detained the rate of cell deaths. This research aims to evaluate the
protective effects of IPC and hypothermia towards morphological changes of liver tissues and the increase of malondialdehyde
(MDA) level as a response to oxidative stress.
Methods:
This experimental study using 24 Oryctolagus cuniculus, consist of four groups of animal, three control animals and
21 experimental animals. The IRI group underwent femoral artery ligation under anesthesia for four hours to induce ischemia.
Afterwards, the ligation was released. The IPC group underwent repeated ligations of right communal femoral artery for two
minutes and three minutes of release in two cycles. Afterwards, the arteries were ligated for four hours. The hypothermia group
underwent ischemia and wrapping of right lower extremities using ice, with temperature around 31-33 ℃. Liver histopathology
and MDA assessment was conducted.
Results:
On histo-morphological assessment, there were histo-morphologic changes on ischemia group compared IPC and
hypothermia (p<0.05).The degree of histo-morphological damage in the IPC group was lower than for the reperfusion ischemia
reperfusion group (p=0.015). MDA levels of IPC group and hypothermia groups were lower than in the ischemic reperfusion
groups (p=0.002).
Conclusion:
Ischemic reperfusion condition causes histo-morphological changes and oxidative stress on liver cells. IPC
and hypothermia have protective effects from ischemia-reperfusion injuries. The protective effects of IPC were better than
hypothermia.
Biography
Sinta Chaira Maulanisa is a General Surgery Resident at dr. Cipto Mangunkusumo National Central General Hospital, Jakarta-Indonesia. She has completed her
Graduation as a Medical Doctor from University of Indonesia. She has done some research about ischemia reperfusion injury. She was worked in rural area of west
java Indonesia as an internship doctor in 2010-2011.
maulanisa.sinta@gmail.comSinta Chaira Maulanisa
et.al., J Gastrointest Dig Syst 2018, Volume 8
DOI: 10.4172/2161-069X-C2-067