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conferenceseries
.com
Volume 8
Journal of Palliative Care & Medicine
ISSN: 2165-7386
Geriatrics 2018
July 30-31, 2018
July 30-31, 2018 | Barcelona, Spain
8
th
International Conference on
Geriatrics Gerontology & Palliative Nursing
In vitro
neuroprotective effect of avocado oil supplementation on neural aged-cells exposed to cortisol
Verônica Farina Azzolin
1
, Ivo Emílio da Cruz Jung
1
, Fernanda Barbisan
1
, Raquel de Souza Praia
2
, Euler Esteves Ribeiro
2
, Ednéa Aguiar Maia Ribeiro
2
,
Marta Medeiros Frescura Duarte
3
and
Ivana Beatrice Mânica da Cruz
1
1
Federal University of Santa Maria, Brazil
2
Amazonas State University, Brazil
3
Lutheran University of Brazil, Brazil
Background & Objective:
Aging brings with it its own age concerns; they are often associated with stress and the development
of emotional issues such as anxiety and depression. Many authors have described stress as a possible trigger for the development
of a series of psychiatric and metabolic pathologies, and the hormone cortisol at high levels seems to be the link between
stress and the development of dysfunctions. In view of the context already presented that older people, due to biological
and psychosocial issues, are more stressed, that stress is a trigger for chronic diseases and that this population in most cases
consumes a great quantity of drugs, it is justified the search for nutritional elements that can contribute to the fight against
stress. One of the potent nutritional elements is a much-consumed fruit in America's avocado (Persea americana). It contains
a rich nutritional matrix with functional properties, which makes it relevant to carry out
in vitro
studies on the potential effect
of supplementation with avocado oil in a stress neuronal model. Therefore, the objective of this study was to evaluate
in vitro
,
the potential neuroprotective effect of avocado against neural aged-cells (SH-SY5Y) exposed to cortisol.
Methods:
The effect of supplementation of SH-SY5Y neural cell culture with avocado pulp oil exposed to cortisol was evaluated.
The following parameters were analyzed in 24 and 72 hours: viability, rate of cell proliferation and variables associated with
oxidative stress and apoptotic markers.
Results:
Avocado showed a protective effect against exposure of neural cells to cortisol. Increasing their viability andproliferation
and reversing apoptosis caused by cortisol, observed in the decline of the protein levels of BAX, BCL-2 and caspase 3 and 8,
as well as the genotoxic effect observed on 8-hydroxy-2'-deoxyguanosine as an increase in antioxidant enzymes such as SOD,
CAT and GPX.
Conclusion:
Despite themethodological
in vitro
protocol limitations, results suggested that avocadooil couldhave neuroprotective
effect against neural aged-cells exposed to cortisol. These data could be relevant to development of a supplement that helps to
decrease stress consequences, mainly in the elderly.
Biography
Verônica Farina Azzolin holds a degree in Biomedicine from the Santo Angelo Higher Education Institute in 2013 and Master's degree in Pharmacology from
Federal University of Santa Maria. She is a PhD student in Pharmacology at Federal University of Santa Maria. She conducts research in the Laboratory of
Biogenomics in the area of toxicogenetics, nutrigenetics and pharmacogenetics. She has experience with cell culture of cancer cells and stem cells, with techniques
of molecular biology, real time PCR, flow cytometry, DNA damage, and biochemical, spectrophotometric and fluorimetric analyzes for the evaluation of oxidative
stress. She has also worked with the
in vivo
model of Drosophila melanogaster with electromagnetic fields. She has experience in the area of clinical analysis.
azzolinveronica@hotmail.comVerônica Farina Azzolin et al., J Palliat Care Med 2018, Volume 8
DOI: 10.4172/2165-7386-C2-018