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Volume 7, Issue 5 (Suppl)
J Palliat Care Med, an open access journal
ISSN: 2165-7386
Geriatrics 2017
September 4-5, 2017
September 4-5, 2017 | Edinburgh, Scotland
Geriatrics Gerontology & Palliative Nursing
7
th
International Conference on
STEM CELL THERAPIES IN PRECLINICAL MODELS OF STROKE: IS THE AGED BRAIN
MICROENVIRONMENT REFRACTORY TO CELLTHERAPY?
Aurel Popa Wagner
a
, Adrian Balseanua
and
Raluca Sandu
a
a
University Medicine of Rostock, Germany
A
ttractive therapeutic strategies to enhance post-stroke recovery of aged brains include methods of cellular therapy that can
enhance the endogenous restorative mechanisms of the injured brain. The translational failure of experimental therapies
in aged subjects might at least partially be related to the aged brain microenvironment. However, in previous work we have
shown that G-CSF alone is effective in improving behavioral recovery after stroke in aged rats. Here, we tested the hypothesis
that treating post-stroke aged rats with the combination of bone marrow-derived mononuclear cells (BM MNC) or bone
marrow-derived mesenchymal cells BM MSC and G-CSF might improve the long term (56 days) functional outcome. To this
end, 1x106 syngeneic BM MSC and BM MNC per kg bodyweight (BW) in combination with G-CSF (50µg/kg, continued for
28 days) were administered via the jugular vein to Sprague-Dawley rats six hours post-stroke. Infarct volume was measured
by magnetic resonance imaging 3 and 48 days post-stroke and additionally by immunohistochemistry at day 56. Functional
recovery was tested during the entire recovery period. Daily G-CSF treatment led to robust and consistent improvement
of neurological function, but did not alter final infarct volumes. The combination of G-CSF and BM MNC, did not further
improve post-stroke recovery. The lack of an additional benefit may be due to a hitherto not well investigated interaction
between both approaches and, to a minor extent, to the insensitivity of the aged brains to regenerative mechanisms. Also
considering recent findings on other tandem approaches involving G-CSF in animal models featuring relevant co-morbidities,
we conclude that such combination therapies are not the optimal approach to treat the acutely injured aged brain.
J Palliat Care Med 2017, 7:5(Suppl)
DOI: 10.4172/2165-7386-C1-012