24 / 24
Page 55
Notes:
conferenceseries
.com
Volume 8
Journal of Gastrointestinal & Digestive System
Gastro Meet 2018
August 06-07, 2018
August 06-07, 2018 Abu Dhabi, UAE
Gastroenterology and Digestive
Disorders
16
th
International Conference on
Transcriptional Regulation of Bile Acid Metabolism and Microsurgery
Shuxin Han
Case Western Reserve University, USA
C
ircadian control of nutrient availability is critical to efficiently meet the energetic demands of an organism. Production
of bile acids (BA), which facilitate digestion and absorption of nutrients, is a major regulator of this process. Here we
identify a KLF15-Fgf15 signaling axis that regulates circadian BA production. Systemic Klf15 deficiency disrupted circadian
expression of key BA synthetic enzymes, tissue BA levels and triglyceride/cholesterol absorption. Studies in liverspecific Klf15-
knockout mice suggested a non-hepatic basis for regulation of BA production. Ileal Fgf15 is a potent inhibitor of BA synthesis.
Using a combination of biochemical, molecular and functional assays (including ileectomy and bile duct catheterization), we
identify KLF15 as the first endogenous negative regulator of circadian Fgf15 expression. Elucidation of this novel pathway
controlling circadian BA production has important implications for physiologic control of nutrient availability and metabolic
homeostasis. Surgery model like intestinal resection is a common therapeutic approach for human diseases such as obesity,
inflammatory bowel disease, Crohn's disease, and colon cancer that often results in severe short bowel syndrome-like adverse
effects including bile acid diarrhea, dehydration, electrolyte disturbances, and nutrient malabsorption. We introduce a murine
ileal resection model, termed ileectomy, to evaluate tissue communication and the maintenance of systemic homeostasis. Bile
is the important cycling fluid in our body composed of multiple components critical for many functions. Here, we successfully
established a murine bile duct catheterization (MBDC) model to collect the pure bile continuously from mice under the
conscious status. These two novel microsurgery models may provide invaluable platforms for various physio-pathological
studies such as ileal regulation of systemic metabolism and diseases, lipid and drug metabolism as well as the diagnosis and
prognosis of human diseases like pancreatic and biliary tract cancers.
Biography
I am currently a Senior Research Associate at Case Western Reserve University in Cleveland, Ohio in the US. I will join as a faculty member at the School of Animal
Science at Anhui Agricultural University in Hefei, Anhui in China this October. I have been engaged in the metabolic biology for thirteen years. The studies broadly cover
the metabolism of endogenous metabolites (e.g., bile acids, steroids, fat, ketone body, and glucose) to exogenous compounds (e.g., drugs). The studies have been
mostly focusing on the transcriptional regulation of metabolism by various transcription factors from previously nuclear receptors to currently developmental transcription
factors. In the past years, I have been dedicating to the study of developmental transcription factor Kruppel-like factor (KLF) family and developed the novel critical fields of
transcriptional regulation of endo- and xenobiotic metabolism under physiological and chemical or surgery induced disease conditions.
sxh569@case.eduShuxin Han, J Gastrointest Dig Syst 2018, Volume 8
DOI: 10.4172/2161-069X-C3-070