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conferenceseries
.com
Volume 7, Issue 4(Suppl)
J Gastrointest Dig Syst, an open access journal
ISSN: 2161-069X
Gastro Congress 2017
September 11-12, 2017
September 11-12, 2017 | Paris, France
12
th
Euro-Global Gastroenterology Conference
The
cagA
and
vacA
genes of
Helicobacter pylori
antibiotics resistance isolated from gastritis and peptic ulcer
pediatric patients in Vietnam
Thi-Ut Nguyen, Thanh–Hai L E
and
Thu-Hien Pham
National Pediatric Hospital, Vietnam
Statement of the Problem:
Helicobacter pylori
(
H. pylori
) antibiotics resistance is the major cause of failure in eradicating
H. pylori
.
Recent studies have showed that the virulence genes associated with
H. pylori
antibiotics resistance have been isolated from adult
peptic ulcer patients. To date, however, there is still lack of evidene about this in Vietnamese pediatric children. The objective of this
study is to evaluate the association between
H. pylori
antibiotics resistance and the presence of
cagA
and
vacA
genes in pediatric
patients.
Methodology &Theoretical Orientation:
150 samples of
H. pylori
isolated from 150 infected pediatric patients whose antimicrobial
susceptibility showed a resistance to at least 1 of 3 antibiotics: amoxicillin, clarithromycin and metronidazole. The
cagA
and
vacA
genes were detected by using multiplex PCR (Polymerase Chain Reaction), between January, 2012 through September, 2013 in
National Hospital of Pediatrics, Hanoi, Vietnam.
Findings:
The result showed that the
cagA
gene was detected in 34%
H. pylori
strains. The rate of vacAs1, vacAs2, vacAm1, vacAm2,
vacAs1/m1, vacAs1/m2 and vacAs2/m2 were 39.3%, 14.7%, 37.3%, 23.3%, 16.7%, 11.3% and 6.6% respectiverly. 8% strains harboured
three virulence genes
cagA
, vacAm1 and vacAs1.
Conclusion & Significance:
The prevalence of
cagA
and the
vacA
alleles distribution didn’t show a significant difference between
susceptible and antibiotics resistance groups. Recommendations therefore, it may not be necessary to administrate different treatment
regimens for children who are infected by antibiotics resistance of
H. pylori
with
cagA
,
vacA
positive.
Biography
Thi-Ut Nguyen is a Pediatrician. She has been working as Gastroenterologist in Gastroenterology Department of the National Pediatric Hospital. She has conducted
several studies on
Helicobacter pylori
antibiotics resistance of gastritis and peptic ulcer among children.
nguyenvuthuyduong2003@yahoo.comThi-Ut Nguyen et al., J Gastrointest Dig Syst 2017, 7:4(Suppl)
DOI: 10.4172/2161-069X-C1-053