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Volume 6, Issue 7(Suppl) J Gastrointest Dig Syst 2016
ISSN: 2161-069X JGDS, an open access journal
Page 31
Notes:
Gastro Congress 2016
October 24-25, 2016
conferenceseries.com
October 24-25, 2016 Valencia, Spain
9th Euro Global
Gastroenterology Conference
Novel in vitro three dimensional culture of human intestinal cell lines to develop a 3D model for
inflammatory bowel disease
Rasha Hatem Saeed Dosh
Sheffield Hallam University
In order to develop an in vitro 3D cell culture model which mimics the natural environment of the small intestine, pNIPAM-
Laponite hydrogel system was investigated. Human colonic adenocarcinoma cell lines: Caco-2 and HT29-MTX have been
widely used in in vitro 3D culture system as these cells have the ability to differentiate into enterocyte-like cells and mucus
producing goblet cells respectively; and exhibit the properties of intestinal epithelia. For these reasons each cell line and co-
cultures were investigated in suspension and layered cultures using the novel pNIPAM hydrogels, cultures were maintained
under static culture or dynamic culture for up to 8 weeks. Cell viability was assessed using Alamar Blue assay, and histological
stains: H&E, Alcian Blue-Periodic Acid Schiffs (PAS) were used to investigate cellular morphological and matrix production.
Scanning electron microscopy (SEM) was also used to assess the morphology of cells within the hydrogel. Both cell types
remained viable and those cultured in layered cultures under dynamic culture formed villus like structures and produced both
acidic and neutral mucins. SEM analysis showed the presence of cells within/on the surface of the hydrogel, where cells formed
circular clusters of cells forming mosaics with each cell having microvilli. We conclude that the pNIPAM-Laponite hydrogel
could provide a novel 3D intestinal in vitro model.
Biography
Rasha Hatem Saeed Dosh has completed her MSc from Al-Mustansiriyah University and worked as a Lecturer at University of Kufa College of Medicine/Iraq. She
has published 4 papers in college of medicine journals. She is currently a second year PhD student at Sheffield Hallam University/UK.
Rasha Hatem Saeed Dosh, J Gastrointest Dig Syst 2016, 6:7(Suppl)