gastro-congress-2016_scientifictracks-abstracts

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Volume 6, Issue 7(Suppl) J Gastrointest Dig Syst 2016
ISSN: 2161-069X JGDS, an open access journal
Page 41
Notes:
Gastro Congress 2016
October 24-25, 2016
conferenceseries.com
October 24-25, 2016 Valencia, Spain
9th Euro Global
Gastroenterology Conference
Long-term restoration of fecal continence after autologous bio sphincter implantation in a large
animal model of passive fecal incontinence
Khalil N Bitar
Wake Forest School of Medicine, USA
Background & Aim: Internal Anal Sphincter (IAS) dysfunction can lead to fecal incontinence (FI). The objective of this study
was to demonstrate sustained restoration of fecal continence using monomeric recordings in a rabbit model of passive fecal
incontinence after implantation of the Bio Sphincter.
Methods: The methodology followed in this study include: (1) New Zealand female white rabbits underwent IAS injury with
hemi-circumferential IAS sphincterectomy; (2) Bio Sphincters were engineered using autologous IAS smooth muscle and
enteric neural progenitor cells. Rabbits were randomized: Control untreated group and Bio Sphincter treated group. In the
treated group, 4 autologous engineered Bio Sphincters were implanted 6-8 weeks following the sphincterectomy surgery; and
(3) Anorectal manometry was used to measure resting anal pressure and recto-anal inhibitory reflex (RAIR) at baseline, 6
weeks post sphincterectomy, and at 3 months and 6 months after Bio Sphincter implantation.
Results: In the control untreated group, deterioration in fecal hygiene, decreased resting tone and loss of RAIR were observed
post sphincterectomy in all rabbits. Autologous Bio Sphincters were successfully implanted into the donor rabbits without
complications. Survival rate was 100%. In the Bio Sphincter treated group, restoration of basal tone (37±3 mmHg, p<0.05) and
RAIR (60±4%, p<0.05) were observed at 3 months was sustained for 6 months following Bio Sphincter implantation.
Conclusions: This study provides proof of concept of safety and efficacy of Bio Sphincters and restoration of IAS integrity and
function and fecal continence in a large animal model. A regenerative medicine approach to restore impaired IAS function
offers a safe and long-term treatment for passive fecal incontinence.
Biography
Khalil N Bitar, PhD,AGAF, is a Professor of Regenerative Medicine, Gastroenterology, Physiology and Biomedical Engineering. He is the Director of Gastroenterology
program at the Wake Forest Institute for Regenerative Medicine. He has published more than 100 papers in high impact journals and has been funded by NIH for
more than 30 years. He is a fellow of the American Gastroenterological Association.
Khalil N Bitar, J Gastrointest Dig Syst 2016, 6:7(Suppl)