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Volume 7, Issue 3 (Suppl) J Gastrointest Dig Syst, an open access journal
ISSN: 2161-069X Gastro 2017
June 12-13, 2017
June 12-13, 2017 Rome, Italy
11th Global
GastroenterologistsMeeting
Esam Elshimi et al., J Gastrointest Dig Syst 2017, 7:3(Suppl)
DOI: 10.4172/2161-069X-C1-050
Over-expressed blood mRNAmatrix metalloproteinase 12: Smart diagnosis and prognosis of HCV-related
hepatocellular carcinoma
Esam Elshimi1, Mostafa Abdel-Samed Mostafa Sakr2, Mohammad Youssery Abdel-Kareem1 and Lobna Mohammad2
1National Liver Institute, Egypt
2Menoufia University, Egypt
Background: Matrix metalloproteinase-12(MMP-12) is involved in tumor invasiveness and metastasis and significantly over-
expressed in tissues of HCC.
Aim: Aim of this study was to investigate the diagnostic and prognostic value of serum mRNA MMP-12 over expression in human
HCC on top of HCV related cirrhosis.
Subjects & Methods: From January 2016 to June 2016, 50 patients with chronic HCV related cirrhosis and HCC, 50 patients with
HCV related cirrhosis compared to 50 healthy persons as a control group. They were selected from National Liver Disease–Menoufia
University; all patients were subjected to tri-phasic CT abdomen when indicated, liver profile, AFP and molecular characterization of
metalloproteinase-12 expression by molecular biology techniques.
Results: There were statistically significant differences between HCC and cirrhotic patients versus control group regarding (CBC
parameters and liver profile), (p-value<0.01), while there were no differences between all groups regarding creatinine (p-value>0.05).
There was a statistically significant difference between HCC patients and other groups regarding mRNA-MMP12 (p-value<0.01),
sensitivity 72.0%, specificity 60.0%, AUC0.68, accuracy 70.9%, P value<0.01, mRNA-MMP12 and/or AFP had sensitivity of 84.0%,
specificity 60.0%, PPV of 51.2, NPP 88.2%. Accuracy of mRNA-MMP12 and/or AFP=68.0%. The sensitivity of mRNA-MMP12 and
AFP= 58.0 %, specificity 78.0%, PPV 56.9%, NPV 78.8% and accuracy 71.3%.
Conclusion: mRNA-MMP12 is a good sensitive, bad specific but accurate in diagnosing HCC. Adding serummRNA-MMP12 to AFP
improves early diagnosis and hence, better prognosis.
Biography
Esam Elshimi is working at Menoufia University, Egypt. He is the recipient of numerous awards for his research works in related fields. His research interests reflect
in his wide range of publications in various national and international journals.