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Volume 6, Issue 6(Suppl) J Clin Toxicol 2016
ISSN: 2161-0495, JCT an open access journal
Page 40
Notes:
Euro Toxicology 2016
October 24-26, 2016
conferenceseries.com
Toxicology & Applied Pharmacology
October 24-26, 2016 Rome, Italy
7th Euro-Global Summit on
Urine sediment as DNA source in the study of susceptibility biomarkers
Pieranna Chiarella, Damiano Carbonari, Pasquale Capone, Delia Cavallo, Sergio Iavicoli, Antonella Mansi, Renata Sisto and Giovanna Tranfo
Istituto nazionale Assicurazione Infortuni sul Lavoro, Monte Porzio, Italy
S everal genes encoding for enzymes involved in xenobiotic biotransformation and oxidative stress response have been
shown to be polymorphic within the human population. These genes are particularly noteworthy in the occupational
toxicology field being useful to identify individuals with higher susceptibility to specific toxic agents, allowing implementation
of measures to reduce the exposure risk. Although blood is the first choice biological matrix to extract genomic DNA, there
are some critical issues hindering its use in occupational studies: 1) blood draw invasiveness 2) difficulty to achieve consent
from donors 3) need of the authorized medical doctor for blood sample collection 4) safety issues during blood processing 4)
time-length of cell isolation procedure. Since urine is the routine sample used in the bio-monitoring of occupational exposure
to chemical agents, the use of the same sample would be advantageous for both purposes, i.e. biomarker determination and
genotyping. Here we report an effective method to isolate genomic DNA from urinary sediment. The protocol is not based on
use of commercial kit, is cheap, rapid and allows determining the genotypic status of individuals using PCR and RFLP analysis.
Before applying this procedure to exposed workers, we tested the efficacy on urine harvested from our institute donors and
carried out genotyping analysis of GSTT1 and GSTM1 enzymes. In conclusion, at least for the genotyping analysis, urine
sediment represents a suitable alternative to whole blood. This approach might be extended further to identify specific gene
and protein biomarkers of dose/exposure in the occupational and environmental setting.
Biography
Pieranna Chiarella has completed her PhD at the Department of Histology and Medical Embryology from Sapienza University of Rome and worked as Post-doc at
the University of Rome Campus Bio-Medico and as Staff Member at the European Molecular Biology Laboratory. She is currently Researcher at INAIL, Department
of Occupational and Environmental Medicine, Epidemiology and Hygiene in Monte Porzio Catone (Rome-Italy).
Pieranna Chiarella et al., J Clin Toxicol 2016, 6:6(Suppl)