euro-toxicology-2016_scientifictracks-abstracts

70 / 89

Volume 6, Issue 6(Suppl) J Clin Toxicol 2016
ISSN: 2161-0495, JCT an open access journal
Page 116
Notes:
Euro Toxicology 2016
October 24-26, 2016
conferenceseries.com
Toxicology & Applied Pharmacology
October 24-26, 2016 Rome, Italy
7th Euro-Global Summit on
Activation of the estrogen receptor by human serum extracts containing mixtures of
perfluorinated alkyl acids from pregnant women’s serum
Christian Bjerregaard-Olesen, Mandana Ghisari and Eva C Bonefeld-Jørgensen
Aarhus University, Denmark
Humans are exposed to a variety of perfluorinated alkyl acids (PFAAs). Several studies have found xenoestrogenic activity
of single PFAAs. Studies on mixture effects of the PFAAs are however sparse. In the present study, we aimed to determine
the xenoestrogenic activity in human serum extracts containing mixtures of PFAAs. Recently, we developed a method to
extract the PFAAs from serum with simultaneous removal of endogenous hormones and interfering steroid metabolites. We
used this method to extract the PFAAs from serum of 397 Danish pregnant women followed by analysis of estrogen receptor
(ER) transactivation using MVLN cells carrying an estrogen response element luciferase reporter vector. Using 17β-estradiol
(E2) concentration-transactivation curves, we calculated the E2-equivalents (EEQ) for the extracts containing the PFAAs. 52%
of the PFAA serum extracts agonized the ER transactivation and 46% enhanced the E2-induced ER transactivation. We found
positive associations between the ER transactivation and the PFAA serum levels. For the relatively few PFAA extracts that
antagonized the ER in the presence of 24 pM E2 (n=38, 10%), we found inverse associations between the ER transactivation
and the PFAA serum levels. The results indicated that the PFAA extracts induced the ER in a non-monotonic concentration
dependent manner. The median EEQ of the extracts containing the PFAAs corresponds to the effect of 0.5 pg E2 per mL serum.
In conclusion, we observed that most of the extracts containing the PFAA mixtures from pregnant women’s serum agonized
the ER and enhanced the E2-induced effects in non-monotonic concentration-dependent manners.
Biography
Christian Bjerregaard-Olesen is defending his PhD thesis entitled, “Perfluoroalkyl acids in serum of Danish pregnant women: Levels, time trends, extraction and ex
vivo xenoestrogenicity” on October 21st 2016 in Deparment of Public Health, Aarhus University in Denmark. Additionally, he has completed his Master’s degree in
Chemistry. He has published six papers in peer-reviewed journals and further four are in preparation.
Christian Bjerregaard-Olesen et al., J Clin Toxicol 2016, 6:6(Suppl)