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conferenceseries
.com
Volume 5, Issue 6(Suppl)
J Infect Dis Ther, an open access journal
ISSN: 2332-0877
Euro Infectious Diseases 2017
September 07-09, 2017
September 07-09, 2017 | Paris, France
Infectious Diseases
6
th
Euro-Global Conference on
Global gene expression in
Escherichia coli
, isolated from the diseased ocular surface of the human eye with a
potential to form biofilm
Ranjith Konduri
LV Prasad Eye Institute, Hyderabad, India
T
he surface of the eye is colonized by several bacteria, which survive as resident or transient commensals. But following trauma
or under immuno-compromised conditions these commensals cause infection of the eye (such as keratitis, endophthalmitis,
orbital cellulitis etc.) often leading to loss of vision. Normally the infection is resolved following treatment with antibacterial agents.
However, over the years many of these organisms have become resistant to drugs due to excessive and indiscriminate use of drugs.
Resistance to drugs may be due to biofilm formation which makes the bacteria impervious to antibiotics. In the present study ocular
E. coli
from patients with ocular infectious disease is used as a model system and was screened for their ability to form biofilm,
antibiotic susceptibility. In addition, to understand the molecular mechanisms underlying the biofilm formation and resistance to
antibiotics in biofilm phase we used DNA microarray. Ten of twelve ocular
E. coli
isolates were resistant to at least one or more of
nine antibiotics tested and majority of isolates were positive for biofilm formation.
E. coli
L-1216/2010 is best biofilm forming isolate
confirmed by confocal and scanning microscopy. Further
E. coli
in the biofilm phase was 100 times more resistant to antibiotics tested
compared to planktonic phase. DNA microarray analysis could differentiate
E. coli
biofilm forming cells from non-biofilm forming
planktonic cells. It was noted that 30% (10.5% up and 19.5% down-regulated) genes were differentially regulated in the sessile biofilm
forming cells compared to the non-biofilm cells. Genes encoding cell adhesion genes, extracellular matrix are upregulated in biofilm
phase. In addition, some of the up-regulated genes encoding antimicrobial efflux virulence, toxin production, and other metabolites
are known to affect the antibiotic susceptibility of planktonic. These genes serve as potential targets for hacking biofilms. This is the
first study on whole genome expression of ocular
E. coli
isolates with a potential to form biofilm. Study on native pathogenic ocular
isolates for biofilm and antibiotic susceptibility is more relevant than type strains which do not necessarily mimic native isolates.
Biography
Ranjith Konduri has done his MSc from the Department of Biotechnology, School of Life Sciences, Pondicherry University, Pondicherry. He has qualified GATE
with percentile of 99.7%. Currently he has registered for PhD with infectious diseases as area of interest. The work involves the identification and functional
characterization of genes involved in antibiotic resistance and biofilm formation. It aims to identify and understand various molecular mechanisms involved in cell
adhesion for the dispersal of the biofilm. Very recently he has published a research article on Biofilm in Journal Gut Pathogens.
kranjith@lvpei.orgRanjith Konduri, J Infect Dis Ther 2017, 5:6(Suppl)
DOI: 10.4172/2332-0877-C1-032