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.com
Volume 5, Issue 6(Suppl)
J Infect Dis Ther, an open access journal
ISSN: 2332-0877
Euro Infectious Diseases 2017
September 07-09, 2017
September 07-09, 2017 | Paris, France
Infectious Diseases
6
th
Euro-Global Conference on
Role of
in vivo
expressed gene candidates for development of molecular and immunological assays to diagnose
pulmonary tuberculosis
Sumedha Sharma
1
, Rakesh Yadav
1
, Ashutosh N Aggarwal
1
, Suman Laal
2
and
Indu Verma
1
1
PGIMER, India
2
School of Medicine, USA
Statement of the Problem:
Tuberculosis (TB) diagnosis is a one of the major areas of interest to control the spread of TB disease
in community. Therefore, there is a need to develop rapid and specific diagnostics easily usable at different health care levels. Our
previous work on mycobacterial gene expression pattern in sputum from pulmonary tuberculosis patients lead to identification
of newer targets, as potential biomarkers. In view of this, the current study was planned to evaluate the role of these candidate
biomarkers in molecular and serodiagnostic tests.
Methodology:
Three of the genes, Rv0986 & Rv0971 along with one Region of Difference (RD) gene Rv3121, were evaluated for
their diagnostic potential in RNA based real time (RT) polymerase chain reaction (PCR). Simultaneously, the peptides from proteins
corresponding to these genes along with five other RD genes were evaluated for their serodiagnostic potential using a peptide based
enzyme linked immunosorbent assay (ELISA) technique.
Findings:
The use of the target genes Rv0986, Rv0971 and Rv3121 in a molecular RNA based assay lead to the detection of smear
positive patients with 100%, 87% and 94% sensitivity and of smear negative TB patients with 50%, 58% and 67% respectively. However,
of all the peptides corresponding to different proteins which were tested in the serodiagnostic ELISA the maximum sensitivity that
could be attained was 37% for smear positive PTB patients and 32% for smear negative PTB patients.
Conclusion & Significance:
A subset of the proteins encoded by the genes expressed by mycobacteria in the sputum have shown less
sensitivity for the development of a serodiagnostic assay, but these genes have shown promising results for the development of a RNA
based molecular assay that can be optimized further after evaluation in a larger cohort of patients.
Biography
Sumedha Sharma started her research career with her dissertation in the Postgraduate degree where she worked on the effect of
Ocimum gratissimum
on the
colon cancer. She qualified various national eligibility test (Indian Council of Medical Research & Council of Scientific & Industrial Research, India) to pursue her
goal in research and academics. Her inclination towards research led her to join the Doctorate Program where her research was focused on tuberculosis (TB).
During her Doctorate Degree, she was selected as a Training Participant in AIDS and TB international training and research program (AITRP) sponsored by
Fogarty International Centre, NIH, USA where she was trained on Microarray Technology. Her microarray work on sputum of PTB patients gave an insight to the
mycobacterial genome specifically expressed in active TB patients, leading to identification of mycobacterial targets, which can be exploit as potential vaccine and
diagnostic candidates.
sumedha.pu@gmail.comSumedha Sharma et al., J Infect Dis Ther 2017, 5:6(Suppl)
DOI: 10.4172/2332-0877-C1-032