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Volume 8, Issue 3 (Suppl) J Clin Exp Dermatol Res, an open access journal
ISSN: 2155-9554 Euro Dermatology 2017
June 07-08, 2017
June 07-08, 2017 Milan, Italy
16th European Dermatology Congress
IL-10+ Breg cells are inversely correlated with IFN-γ+ NK cells and IL-17+ NK cells in psoriasis and
psoriatic arthritis
Lazaros I Sakkas, Areti Varna, Christos Liaskos, Efterpi Zafiriou, Marianna Vlychou, Christina Katsiari, Dimitrios P Bogdanos and Athanasios Mavropoulos
University of Thessaly, Greece
Background & Purpose: Recently we reported functional impairment and numerical decrease of transitional and memory IL-
10-producing regulatory B cells (Bregs) in patients with psoriasis (Ps) and psoriatic arthritis (PsA). In this study we explore the
relationship of IL-10+Breg cells with NK subsets expressing the IFN-γ and IL-17 pro-inflammatory cytokines.
Methods: Peripheral blood mononuclear cells from 40 PsA, 40 Ps patients and 20 healthy controls (HCs) were studied. Flow
cytometric analysis was carried out with MoAbs against cell surface markers CD56, CD16, CD3, CD7, CD19, CD24, CD27 and
CD38. Intracellular expression of cytoplasmic IFN-γ, IL-17 and IL-10 following bacterial CpG (ODN2006) and PMA/ionomycin
stimulation was also examined by flow cytometry.
Results: The percentages of CD3-CD56+ (NK) inversely correlated with CD19+CD24hiCD38hi (transitional) Bregs and
CD19+CD24hiCD27+ (memory Bregs) (p<0.05, for both). IFN-γ+ NK cells negatively associated with IL-10+Bregs. Also, IL-17+NK
cells inversely correlated with IL-10+Bregs (p<0.05). IFN-γ or IL-17 production from NK cells was significantly higher in PsA and
Ps compared to HCs (p<0.05 for both).
Conclusion: Deficient IL-10-producing Breg cells are associated with an increase of IFN-γ- and IL-17-producing NK cells in Ps and
PsA suggesting an important pathogenetic role of pro-inflammatory innate immune cells in these diseases.
Biography
Lazaros I Sakkas is working as a Professor of Medicine and Rheumatology, Chairman, Department of Rheumatology and Clinical Immunology, Faculty of Medicine,
School of Health Sciences, University of Thessaly. He is also an Adjunct Assistant Professor, Temple University School of Medicine, Philadelphia, PA, USA (Jun
2003-present) and an Adjunct Professor, Center for Molecular Medicine, Old Dominion University, Norfolk, VA, USA (March-2010-March 2017). He is the President
at the Institute for Rheumatic Diseases (2011-present).
Lazaros I Sakkas et al., J Clin Exp Dermatol Res 2017, 8:3 (Suppl)
DOI:10.4172/2155-9554-C1-056