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Volume 8 Journal of Alzheimers Disease & Parkinsonism
ISSN: 2161-0460 Euro Dementia 2018
May 24-25, 2018
May 24-25, 2018 | Vienna, Austria
11th International Conference on
Alzheimers Disease & Dementia
Development and progression of Alzheimer’s disease in Sprague-Dawley rats administered with
streptozotocin intrahippocampally
Gurmeet Kaur Surindar Singh1, 2, Mazzura Wan Chik1 and Nurul Aqmar Mohd Nor Hazalin 2
1Universiti Teknologi MARA (UiTM)—Puncak Alam Campus, Malaysia
2Universiti Teknologi MARA (UiTM)—Shah Alam Campus, Malaysia
Alzheimer’s disease (AD) rat model can be reproduced via intrahippocampal (IH) administration of streptozotocin (STZ)
in the rat’s brain. The hippocampus holds a large amount of insulin receptors (IRs) which are very sensitive to STZ. IRs’
exposure to STZ prompted memory impairment and production of amyloid-beta plaques related to AD pathogenesis. The
present study is conducted to investigate the effects of IH-STZ administration on the progression of memory impairment and
formation of amyloid β (Aβ) at 3, 6 and 12 weeks of STZ-treatment. Sixty male Sprague-Dawley rats (350–450 g) were divided
into groups of control (no treatment), sham-operated (received PBS) and IH-STZ treated (G3w, G6w and G12w). STZ (3 mg/
kg; 5 µl) was administered bilaterally as a single injection into the dorsal hippocampus of the rats. The memory impairment
was studied a week before decapitation using Morris water maze test. The rats were sacrificed at week 3, 6 and 12 after STZ
administration and presence of Aβ plaques were studied using the immunohistochemistry. All IH-STZ rats showed significant
results in escape latency, total distance travelled and swimming speed (p<0.05) when compared to sham indicating memory
impairment. In conclusion, STZ when injected intrahippocampally, developed memory impairment as early as two weeks after
STZ treatment in the rats.
J Alzheimers Dis Parkinsonism 2018, Volume 8
DOI:10.4172/2161-0460-C3-043