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.com
Volume 9
Journal of Clinical & Experimental Cardiology
ISSN: 2155-9880
October 22-24, 2018 | Rome, Italy
27
th
European Cardiology Conference
Euro Cardiology 2018
October 22-24, 2018
Different proteomic approaches identifies molecular networks underlying cardiac remodelation in
western diet-induced obese rats
Danielle Fernandes Vileigas
1
, Paula G Sant’Ana
1
, Paula P Freire
2
, Dijon H S de Campos
1
, Katashi Okoshi
1
, Victoria M Harman
3
, Lucilene D dos Santos
1
,
Robert J Beynon
3
and
Antonio C Cicogna
1
1
Botucatu Medical School – UNESP, Brazil
2
Bioscience Institute - São Paulo State University, Brazil
3
University of Liverpool, UK
O
besity is a complex disease state that is often associated with pathologic changes in the heart, impairing both diastolic and
systolic function. Previous studies has investigated potential contributors to this dysfunction, however, the adverse effects
of obesity on cardiac function remain incompletely understood. The aim of this study is to identify the myocardial proteins that
are differentially expressed between obese rats with cardiac dysfunction and healthy controls, using two different proteomic
approaches. Male Wistar rats were distributed into two groups: control (n=13; standard diet) and obese (n=13; Western
diet) fed for 41 weeks. The obesity was determined by adipose index. Cardiac function was evaluated by echocardiogram
and isolated papillary muscle analysis. The proteomics was based on two-dimensional gel electrophoresis (2DE) followed by
mass spectrometry identification (LC-MS/MS) and nano-liquid chromatography with tandem mass spectrometry (nanoLC-
MS/MS) followed by label-free quantification. The differentially expressed proteins were subjected to enrichment analysis
using the DAVID bioinformatic tool. Obese rats showed increased adiposity index (p<0.001). Echocardiographic assessment
revealed decreased ejection fraction (p=0.029) in obese group. Papillary muscle evaluation indicated both diastolic and systolic
dysfunction in baseline condition and in post-rest potentiation maneuver in obese group. A total of 87 myocardial proteins
were identified as differentially expressed between control and obese groups, being 46 up- and 41 down-regulated, respectively,
in the obese group. Proteins with increased expression are involved in several important biological processes including
mitochondrial and peroxisomal fatty acid beta-oxidation, lipid homeostasis (transport and catabolism), oxidative stress
pathways and regulation of cardiac muscle contraction by calcium ion signaling. Proteins associated with the cytoskeleton
were also elevated. The proteins of lower expression were predominantly from pathways involved in defense against oxidative
stress, as well as in glycolysis and amino acid metabolism, tricarboxylic acid cycle, respiratory electron transport chain, ATP
metabolic process and cardiac contraction. In conclusion, these two complementary proteomic approaches revealed several
molecular alterations in the myocardium of obese rats, enabling a better understanding of the molecular mechanisms involved
in cardiac dysfunction, which may suggest some potential novel therapeutic targets for treatment and/or prevention of heart
complications in obesity.
Biography
Danielle F Vileigas obtained her BSc Degree in Nutrition (2010) and MSc Degree in Pathophysiology in Internal Medicine (2015) from the São Paulo State
University, Brazil respectively. Currently, she is pursuing her PhD student at the same university and has gained an International Fellowship from São Paulo
Research Foundation to pursue a research internship during her PhD for 4 months at the Centre for Proteome Research of the University of Liverpool, UK. Her
current research involves proteomic approaches to understanding the molecular mechanisms underlying cardiac dysfunction in obesity. She worked on several
projects in obesity and cardiology fields.
Danielle Fernandes Vileigas et al., J Clin Exp Cardiolog 2018, Volume 9
DOI: 10.4172/2155-9880-C10-116