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Volume 9
Journal of Clinical & Experimental Cardiology
ISSN: 2155-9880
October 22-24, 2018 | Rome, Italy
27
th
European Cardiology Conference
Euro Cardiology 2018
October 22-24, 2018
Thoracic aortic aneurysm and age-related vascular remodeling: Study of epigenetic mechanisms
Vaiva Patamsyte
1
, Dovydas Gecys
1
, Giedrius Zukovas
1
, Stase Gasiule
2
, Vaidotas Stankevicius
2
, Giedrius Vilkaitis
2
, Rimantas Benetis
1
and
Vaiva
Lesauskaite
1
1
Lithuanian University of Health Sciences, Lithuania
2
Institute of Biotechnology - Vilnius University, Lithuania
T
he key players in age-related vascular remodeling are vascular smooth muscle cells (VSMCs). Senescent VSMCs are
phenotypically shifted from contractile into the secretory phenotype. Same phenomenon occurs during morphogenesis
of dilatative pathology of ascending aorta. We tested if miR-21-5p, miR-143-3p, and miR-145-3p expression levels are similar
during formation of thoracic aortic aneurysm (TAA) and in aging aorta. Expression of miRNAs was evaluated in aortic tissue
specimens from TAA patients (N=8), donors and coronary artery bypass surgery (CABG) patients younger than 55 years
(N=7), and CABG patients older than 70 years (N=8) using qRT-PCR. ΔCt values for each miRNA were calculated using
miR-16-5p and miR423-5p as reference. A significant increase in miR-21-5p expression was found in TAA patients compared
to younger individuals (p=0.04). Even a stronger difference was observed when young individuals were compared with older
individuals (p=0.03). There was no significant difference in miR-21-5p expression between TAA patients and older individuals
(p=0.05). These results show that similar miR-21-5p expression profiles can be identified in dilated and aging aortic tissue.
A strong association was found between aortic diameters of TAA patients with bicuspid aortic valve (BAV) and miR-21-5p
expression (r= 0.821, p=0.02). Same tendency was observed between age and miR-21-5p expression in individuals without
TAA (r= 0.576, p=0.02). We did not find a significant difference in miR-143-3p and miR-145-3p expression among the three
study groups (p>0.05). Our results show that epigenetic mechanisms involved in age related vascular remodeling are similar to
the pathological processes which occur during formation of TAA.
Biography
Vaiva Patamsyte pursued her BSc in Genetics and MRes Biosciences from Cardiff University, UK and is currently a PhD student at the Lithuanian University of
Health Sciences, Lithuania. She has been working in the Laboratory of Molecular Cardiology for the last four years. Her research focuses on genetic and epigenetic
regulation of vascular remodeling.
Vaiva Patamsyte et al., J Clin Exp Cardiolog 2018, Volume 9
DOI: 10.4172/2155-9880-C10-116