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Volume 7, Issue 3(Suppl)

J Biotechnol Biomater, an open access journal

ISSN: 2155-952X

Euro Biotechnology 2017

September 25-27, 2017

Page 11

conference

series

.com

17

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EURO BIOTECHNOLOGY CONGRESS

September 25-27, 2017 Berlin, Germany

Elizabeth E Hood, J Biotechnol Biomater 2017, 7:3(Suppl)

DOI: 10.4172/2155-952X-C1-075

Production of industrial enzymes in maize

I

ndustrial enzymes are excellent technologies to apply in manufacturing to alleviate environmental pollution. Using plant-

based materials allows manufacturing of goods from renewable resources. My laboratory and company are engaged in

producing enzymes for industrial applications using the plant seed system. The advantages of the seed system are that the

production costs can be quite low because scaling-up just involves planting more acres, the seed can be stored for years,

and the enzymes are extremely stable in the seed. We use maize grain to express enzyme genes from fungal and bacterial

sources that have specific applications in biofuels, bioproducts and waste water remediation. Genes for these enzymes are

partially codon optimized and their expression is driven by either an embryo or an endosperm promoter and targeted to

one of three subcellular locations—the cell wall, the endoplasmic reticulum or the vacuole. Expressed enzymes include three

cellulases, a phospholipase, a Mn peroxidase, and a laccase. Once the gene is expressed and a high-expressing event chosen,

breeding into elite inbred germplasm commences. Applications of the various enzymes will be discussed along with regulatory

considerations.

Biography

Elizabeth E Hood has 35 years of experience in Biology. She is Distinguished Professor of Agriculture at Arkansas State University and; CEO of two biotechnology start-up

companies—Infinite Enzymes, LLC and Infinite Eversole Strategic Crop Services, LLC. Previously, she was an Associate Vice Chancellor for Research and Technology

Transfer at ASU; Program Director in Molecular and Cellular Biosciences at the National Science Foundation: Leader in forming one of the world’s foremost transgenic

plant research groups at ProdiGene, a plant biotechnology company. She has completed his PhD in Plant Biology at Washington University and MS in Botany awarded

by Oklahoma State University.

ehood@astate.edu

Elizabeth E Hood

1,2

1

Arkansas State University, USA

2

Infinite Enzymes, LLC, USA