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Volume 6, Issue 7(Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Page 41
Euro Biotechnology 2016
November 07-09, 2016
conferenceseries.com
November 07-09, 2016 Alicante, Spain
12th Euro Biotechnology Congress
D H Tejavathi, J Biotechnol Biomater 2016, 6:7(Suppl)
Novel in vitro techniques for sustainable cultivation and efficient induction of genetic variability in
Agave vera-cruz Mill
Agave vera-cruz Mill is a member of Agavaceae, is one of the important fiber yielding, ornamental and medicinal plants.
Gradual depletion of genetic resources of economically important plants is the result of indiscriminate collection of the
source material and disappearance of natural habitats due to human intervention. At this juncture, plant genetic improvements
programs are primarily dependent on the availability and efficient induction of genetic variability. Novel techniques like in vitro
culture, in vitro mutagenesis and AM fungal association provide a scope for induction of much needed genetic variability in the
base populations. Nearly 100 shoots were differentiated from callus raised from shoot tip cultures on transferred to ½MS+BAP.
Among the regenerated plants, a few plants raised through indirect organogenesis have shown a few phonotypic variations
from the source plants. Shoot tips were exposed to EMS at various concentrations and α-irradiation for varying periods and
doses. It was found that multiple shoot induction from these cultures was two-fold more than the control plants. Normal and
tissue culture plants were treated with Glomus mosseae and G. fasiculatum; two AM fungal species to study their effect on the
enhancement of the biomass and active principles. The increase in biomass was found to be threefold than the control plants.
Effect of in vitro mutagenesis and AM fungal association on the synthesis of primary and secondary metabolites in control
and treated plants were studied. Finally, genetic variability induced by these novel techniques in micro-propagated plants was
analyzed by AFLP markers.
Biography
D H Tejavathi is working as UGC BSR-Faculty Fellow in the Department of Botany, Bangalore University, India. She has published 80 research articles in various
national and international journals and completed 8 research projects funded by DST, CSIR, BU-UGC and MoEF, India. She was conferred with an award ‘Merit
of Excellence’ for outstanding contribution to the medicinal plant research during the 4th international conference on medicinal plants and herbal products held at
John Hopkins University, USA, 2012.
D H Tejavathi
Bangalore University, India