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Volume 7, Issue 1 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Enzymology & Mol. Biology 2017
Biotechnology Congress 2017
March 20-21, 2017
Page 55
Notes:
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March 20-21, 2017 Rome, Italy
&
15th World Congress on
2nd International Conference on
Biotechnology And Biotech Industries Meet
Enzymology and Molecular Biology
Uranium bio-precipitation and recovery from high radiation environments: New approaches
Removal of traces of uranium from nuclear waste poses a big challenge for its disposal. Our laboratory has genetically
engineered the extremely radio-resistant bacterium Deinococcus radiodurans to over-express either an acid phosphatase
PhoN, or an alkaline phosphatase PhoK, to achieve impressive uranium bio-precipitation (up to 7-10g U/g dry biomass) over a
wide pH (5-9) and uranium concentration (0.2-10 mM) range. Successful preservation of bioprecipitation-active dry biomass
for up to 2 years at ambient temperature has been achieved. Conditions have been optimized to accomplish easy and complete
recovery of precipitated uranium. Further augmentation of uranium bioremediation has been accomplished by: pyramiding
phoN and phoK genes in a single strain, employing radiation-responsive Deinococcus gene promoters, and by surface display
of bioremediation-active enzymes.
Biography
Shree Kumar Apte is the Former Director, Bio-Science Group, BARC and currently serves as a Professor at the Homi Bhabha National Institute. He is a JC Bose
National and Raja Ramanna Fellow at BARC, Mumbai, India. His laboratory has unraveled stress and adaptive responses of several bacteria and developed many
biotechnologies for metal bioremediation from high radiation environments. He is a fellow of all National Science Academies and Agriculture Academy in India.
Shree Kumar Apte
Homi Bhabha National Institute, India
Shree Kumar Apte, J Biotechnol Biomater 2017, 7:1(Suppl)