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.com
Volume 7, Issue 2 (Suppl)
J Ecosyst Ecography, an open access journal
ISSN:2157-7625
September 18-20, 2017
September 18-20, 2017 Toronto, Canada
Joint Conference
International Conference on
International Conference on
Environmental Microbiology and Microbial Ecology
&
Ecology and Ecosystems
Immobilization of murine noroviruses in a cultivated phaeozem soil and its reversibility
Vincent Tesson
1
, Gael Belliot
2
, Line Capowiez
1
and
Pierre Renault
1
1
INRA, France
2
University of Dijon, France
W
astewater reuse in irrigation may contaminate soils with human enteric viruses that can be internalized in plants via their
roots. Experiments have shown that virus immobilization in soils varies with virus, soil and soil solution; results were mostly
obtained at saturation and the reversibility of immobilization was not clearly assessed. Models generally use a partition coefficient,
although colloid immobilization is often assumed kinetic and non-reversible. Our objectives were to explore other contexts, assess the
reversibility of immobilization and the relevance of equilibriumor kineticmodels. Columns of phaeozemsoil aggregates were saturated
with soil solutions optionally enriched with MgCl
2
or fulvic acids, under vacuum or air to affect air trapping. For immobilization,
the solutions circulated at different temperatures between the columns and reservoirs contaminated with murine noroviruses. For
remobilization, contaminated columns were stored between 0 and 6 days at some moistures and then rinsed by - virus-free - soil
solutions, sterilized wastewater or underground water. Solutions were sampled in the reservoirs or at the column outlets; half aliquots
were filtered at 0.45 µm. Viruses retained in soils were extracted. Immobilizations were also monitored on stirred soil suspensions.
Viruses were quantified by RT-qPCR and cell culture. The relevancies of models combining flow and immobilization were evaluated.
Except after MgCl
2
enrichment, the weak virus immobilization over 1 day - i.e. about 40% of the brought viruses – could be quantified
only from remobilization experiments. When saturated conditions are kept for 6 additional days, most of viruses are progressively
immobilized, only a few being remobilized later with virus free soil solution. Fulvic acids increase virus remobilization. By contrast,
the partial drying of soil columns after the initial immobilization day increases the irreversibility of the immobilization. Models
considering inter- and intra-aggregate pore spaces as well as reversible and irreversible virus immobilizations fitted to experiments.
Biography
Vincent Tesson has his expertise in the environmental fate of human enteric viruses. After working on the detections of equine and
Bovine coronavirus
by RT-qPCR
and FTIR spectroscopy, respectively, he undertook a Ph.D on the environmental fate of human enteric viruses by analyzing their fate after wastewater discharge
in river or reuse in agricultural irrigation. He has completed a work on the fate of viruses in the soil that contributes to a project on green onion contamination. He
is currently working on the simulation of virus discharge in rivers from regional acute gastroenteritis epidemiological data.
vincent.tesson@inra.frVincent Tesson et al., J Ecosyst Ecography 2017, 7:2 (Suppl)
DOI: 10.4172/2157-7625-C1-029