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Volume 7, Issue 2 (Suppl)
J Ecosyst Ecography, an open access journal
ISSN:2157-7625
September 18-20, 2017
September 18-20, 2017 Toronto, Canada
Joint Conference
International Conference on
International Conference on
Environmental Microbiology and Microbial Ecology
&
Ecology and Ecosystems
Insight into the diversity of soil fungi in Changbai mountain by high-throughput sequencing
Guozhong Lyu
1
, Dongning Shi
1, 2
, Zhihui Zhao
1
and
Hong Yang
1
1
Dalian Nationalities University, China
2
Liaoning Normal University, China
S
oil fungi are an important group of microorganisms in forest ecosystem, they play pivotal roles in cycling of organic compounds
and further affect the below-ground and above-ground ecosystems. In contrast to soil bacteria, soil fungi have been poorly
understood in forest ecosytem. The rapid development of molecular technologies offers an effective method to access more functional
information on soil fungal diversity. We applied the technique of Illumina Miseq High-Throughput Sequencing to investigate the soil
fungal diversity and community structures in the northern slope of Changbai Mountain, Jiling Province, China, which is characterized
with an evident vertical vegetation distribution pattern along with the altitude. The metagenome sequence analysis was conducted by
targeting ITS1f-ITS2 fragments for 80 soil samples collected in the four characteristic forest vegetation bands ranging from the root of
700 m to the top of 2600 m in altitude, it reveals a tremendous abundance of soil fungi in Changbai mountain forest. Totally 2,294,552
rDNA fragments of reads are grouped into 25,282 operational taxonomic units (OUTs), they belong to 1056 species, 622 genera, 195
families, 87 orders, 24 classes and 5 phyla of fungi. Among which 182 genera are of
Basidiomycota
(48.72%), 411 genera of
Ascomycota
(31.67%), 13 genera of
Zygomycota
(10.21%), 13 genera of
Chytridiomycota
(0.27%), 3 genera of
Glomeromycota
(0.04%), and the left
9.09% are unclassfied taxa. The species of
Basidiomycota
are the predominant compents of the soil fungi in the mountain, especially
the genera of
Laccaria
(6.17%),
Inocybe
(5.54%),
Hygrocybe
(3.06%), and
Russula
(2.37%) of Agricales. While the genera of
Mortierella
(6.73%) and
Inocybe
are most widely distributed in all altitude sampling soils in the mountain. The soil fungal richness evidently tends
to decrease from the root to the top of the mountain, and the fungal compositions vary in the four characteristic vegetation bands of
the mountain. The novel profile of soil fungi in the mountain uncovered by means of metagenome technique could not be obtained
by conventional fungal research methods.
Biography
Guozhong Lyu has completed his Ph.D from Shenyang Agricultural University. He is the Dean of College of Environment and Resources, Dalian Nationalities
University, China. He has published 150 papers mainly about fungal biodiversity, plant diseases and biocontrol of invasive weeds in academic journals.
lvgz@dlnu.edu.cnGuozhong Lyu et al., J Ecosyst Ecography 2017, 7:2 (Suppl)
DOI: 10.4172/2157-7625-C1-029