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.com
Volume 7
Biosensors Journal
ISSN: 2090-4967
Electrochemistry 2018
June 11-12, 2018
June 11-12, 2018 | Rome, Italy
4
th
International Conference on
Electrochemistry
Enzyme engineering in bioelectrochemical systems
Lital Alfonta
Ben Gurion University of the Negev, Israel
C
oupling between redox enzymes and electrodes relies first and foremost on a successful interfacing between the enzymes
and the inorganic surfaces with which they interact. An interface that allows from one hand a specific analyte recognition
and on the other hand an efficient signal transduction. Some of the challenges in interfacing between biological molecules
and inorganic materials stem from wrong orientation of the enzyme towards the interface and from the need to use mediated
electron transfer with a diffusional redox mediator due to a difficulty in relaying a signal from a redox center that is deeply
buried inside the protein matrix. Using genetic code expansion tools, and genetic engineering approaches we were able to
modify redox enzymes and surfaces for biosensing and biofuel cell applications so they could have superior properties over
native enzymes. In my talk, I will demonstrate how does site-specific wiring of redox enzymes which is genetically encoded,
can improve electron transfer due to controlled and short electron transfer distances and due to proper enzyme orientation. I
will also demonstrate how a rational genetic engineering of an enzyme gives it superior properties and activity, compared to
those of the native enzyme.
alfontal@bgu.ac.ilBiosens J 2018, Volume 7
DOI: 10.4172/2090-4967-C1-003