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.com
Volume 2, Issue 2 (Suppl)
Dent Implants Dentures, an open access journal
ISSN: 2572-4835
Dental Medicine 2017
October 16-18, 2017
October 16-18, 2017 New York, USA
29
th
Annual World Congress on
Dental Medicine & Dentistry
Efficacy of vascular endothelial growth factorandMTAon angiogenesis of dental pulp stemcells transferred
onto polymeric scaffold
Mohammad Samiei, Marzieh Aghazadeh, Oldouz Hosseinighalehjough and Nadia Jahedjou
Tabriz university of Medical Sciences, Iran
Introduction:
Stem cells are considered to have self-renewal and multipotential entities and thus can generate several differentiated
cell types and regenerating tissues. Vascular endothelial growth factor (VEGF) plays a central role in angiogenesis, promoting
the formation of new capillaries. Mineral trioxide aggregate (MTA) is a biocompatible material that has a wide range of clinical
applications, including pulp capping, root perforation repair, and root-end filling. This study investigated effect of VEGF and MTA
on angiogenesis of dental pulp stem cells transferred onto polymeric scaffold.
Materials &Methods:
Dental pulp stem cells have been prepared in a form of equipped and increased to 2 million cells. After passing
the preparation procedure, polymeric wells that made by poly caprolactone chitosan (PCL-CS) polymer, inserted in plates and 105
cells transferred to the palates. VEGF was added to the chambers of the experimental groups. After 14 days of incubation, the cells
were transferred to flowcytometry center for assessment of CD31 and VEGFR2 as the angiogenesis factors.
Results:
In the PCL-CS-MTA scaffold group, in the presence of VEGF, human dental pulp stem cells can express 90+8% of VEGFR2.
While the expression of this receptor on cells cultured on PCL-Cs scaffold alone was 1/2+0/9%. Furthermore, the CD31 receptor
expression was 71/4± 6/6% on the PCL-Cs-MTA, however, CD31 expression in control group was 5±1/2%. The increase in both
receptor expression was statistically significant (p<0.05).
Conclusion:
The present study shows that angiogenesis of dental pulp stem cells is increased in the presence of VEGF on the polymeric
scaffold with MTA. Consequently, it seems that VEGF and MTA have the ability of enhancing angiogenesis in pulp stem cells.
Biography
Mohammad Samiei is an Assistant Professor of Endodontics at Tabriz University of Medical Sciences. He has a great experience in the field of Dentistry. He is a course
Director, Lecturer and Instructor in Department of Community Dentistry.
Samiei.moh@gmail.comMohammad Samiei et al., Dent Implants Dentures 2017, 2:2 (Suppl)
DOI: 10.4172/2572-4835-C1-003