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Volume 8

Journal of Alzheimers Disease & Parkinsonism

ISSN: 2161-0460

Dementia 2018

October 29-31, 2018

October 29-31, 2018 | Valencia, Spain

12

th

International Conference on

Alzheimer’s Disease & Dementia

Amyloidogenesis under experimental neurodegeneration in rats

Zagrebin V L

1

, Yermilov V V

1

, Zub A V

1

, Antoshkin O N

2

, Sagrsyan S A

1

and

Lipov D S

1

1

Volgograd State Medical University, Volgograd, Russia

2

Clinical Hospital of The Presidential Administration, Moscow, Russia

Introduction:

One of the mechanisms in the pathogenesis of proteinopathy is the formation of two types of histopathological

inclusions: senile plaques with the main role of β-amyloid peptides formed during specific cleavage of amyloid precursor

protein (APP), and neurofibrils formed by pathological forms of protein. Both of these components are currently considered

as potential molecular targets, the impact of which will allow to slow down or stop the development of the neurodegenerative

diseases.

Aim:

The aim of the work was to identify amyloid proteinopathy in the cerebral cortex during experimental neurodegeneration.

Materials &Methods:

The study was performed on 20 white male rats, 24 months of age. The physical and process stress effect

was reproduced for seven days.

Discussions & Results:

Neurons of experimental group had a predominantly large bright, almost transparent nucleus,

occupying more than half of the cytoplasm. The nuclei were eccentrically located with segregated nucleoli of irregular elongated

shape. The basophilic substance was practically not detected in the cytoplasm when stained with thionin. Neurons with clear

signs of apoptosis were noted. Congo red staining for amyloid revealed intracellular inclusions. Extracellular fibrillar structures

were also found. In the group of intact animals, chromatin in the nucleus was clearly pronounced and a sufficient amount of

basophilic substance was observed in the perikaryon.

Conclusions:

Neurodegenerative disturbance in neurons of the cerebral cortex are characterized by apoptosis and proteinopathy

with a pronounced predominance of intracellular and extracellular amyloid.

Biography

Zagrebian V L completed his PhD from the Volgograd State Medical University. Later he become the Vice Dean of Medical Faculty and was elected as Head of

the Department of Histology, Embryology and Cytology of Volgograd State Medical University. He was elected as President of the Federation of Youth Scientific

Societies of Medical Universities of Russian Federation. He is also an Associate Professor, who has published more than 150 papers in reputed journals and has

been serving as an Issuing Editor of a scientific journal of Volgograd State Medical University.

vlzagrebin@gmail.com

Zagrebin V L et al., J Alzheimers Dis Parkinsonism 2018, Volume 8

DOI: 10.4172/2161-0460-C7-054