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Volume 8

Journal of Alzheimers Disease & Parkinsonism

ISSN: 2161-0460

Dementia 2018

October 29-31, 2018

October 29-31, 2018 | Valencia, Spain

12

th

International Conference on

Alzheimer’s Disease & Dementia

Ionizing radiation for awakening LPS treated BV-2 microglia cells

Weonkuu Chung, Mi Ju Chung, Dong Wook Kim, Chan Woo Kim, Jong Kil Lee, Hak Young Lee, Geon-Ho Jahng

and

Se Young Choi

Kyung Hee University Hospital at Gangdong, Seoul, South Korea

T

he aim of this study was to investigate the mobility of microglia when irradiated with low dose radiation (5.4 Gy/3 fr and

9 Gy/5 fr) after inhibiting by high dose lipopolysaccharide (LPS) in microglia cells. BV-2 microglia cells were treated with

various concentrations of LPS to determine the optimal concentration to inhibit their mobility and the change of microglia’s

mobility was defined with wound healing assay. The same experiment was conducted to confirm the effect of low dose

irradiation on microglia’s mobility after treating with LPS. As a result, we saw that 2 μg/mL of LPS suppressed the mobility

of movement distance greatly for microglia cells to 2.96 μm. So, then we selected a 2 μg/ml of LPS for BV-2 cells as optimal

concentrations. We found that LPS (2 μg/mL) inhibited microglia cells mobility significantly to 1.62 μm (p<0.001). Compared

with the control group and the irradiated group, the control group and the RT5 group (9 Gy/5 fr) showed similar median

movement distances (12.5 μm vs., 15.6 μm p=0.98). That means 9 Gy radiation dose didn’t inhibit mobility of microglia cells.

But we couldn’t see that 5.4 Gy/3 fr group made enhanced effect for mobility of microglia cells. When the control group and the

LPS+RT5 (9 Gy/5 fr) group were compared, we could define ionizing radiation make enhance median mobility from inhibited

status to as like normal condition. (control G 13.79 μm vs., LPS + RT5 12.14 μm, p=0.84). In Summary, the activity of BV-2

cells was reduced by high dose LPS (2 μg/mL). It was also confirmed that the migration of suppressed BV-2 cells was similar to

that of the control group when treated with low doses (9 Gy/5 fr) of radiation. We have found that low dose irradiation is one

of the ways to increase the activity of microglia cells and we will continue to enhance mobility of microglia cells. If we know

that ionizing irradiation can control microglia activity, we can use a radiation treatment for neurodegenerative disease such as

Alzheimer’s disease.

Biography

Weonkuu Chung did his Research Fellowship in Radiation Oncology Biology Lab, Duke Medical School. Durham, USA during March 1999- February 2000. During

April 2009-February 2010 he worked as Professor in Radiation Oncology, Konyang University Hospital. From March 2010, he is working as Professor in Radiation

Oncology, Kyung Hee University Hospital at Gangdong. He is a regular member of The Korean Society for Radiation Oncology, ESTRO and ASTRO.

Wkchung16@gmail.com

Weonkuu Chung et al., J Alzheimers Dis Parkinsonism 2018, Volume 8

DOI: 10.4172/2161-0460-C7-054

Notes: