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Volume 7, Issue 6 (Suppl)
J Gastrointest Dig Syst, an open access journal
ISSN: 2161-069X
Page 61
December 07-08, 2017 Madrid, Spain
&
13
th
International Conference on Clinical Gastroenterology & Hepatology
2
nd
International Conference on Digestive Diseases
CO-ORGANIZED EVENT
Comparison of oral and intestinal human microbiota and association of
Fusobacterium nucleatum
infection in patients with colorectal cancer: Apilot study
Edda Russo
1
, Giovanni Bacci
1
, Carolina Chiellini
1
, Camilla Fagorzi
1
, Elena Niccolai
1
, Antonio Taddei
1
, Federica Ricci
1
and
Amedeo Amedei
1,2
1
University of Florence, Italy
2
Azienda Ospedaliera Universitaria Careggi (AOUC) Florence, Italy
T
he study used next-generation sequencing (NGS) to analyze and compare human microbiota from three different
environments, saliva, feces, and cancer tissue (CT), of a selected cohort of 10 Italian patients with colorectal cancer
(CRC) vs. 10 healthy controls (saliva and feces). Furthermore, the
Fusobacterium nucleatum
(
F. nucleatum
) abundance in
the same districts was investigated trough quantitative polymerase chain reaction (RT-qPCR) to assess the association with
CRC. The difference of bacterial taxonomic composition,
F. nucleatum
abundance between CRC and healthy controls and the
relationship of
F. nucleatum
presence with clinical variables were evaluated. Taxonomic analysis revealed the presence of three
main bacterial phyla, which comprises ca. 80% of reads: Firmicutes (39.18%), Bacteroidetes (30.36%), and Proteobacteria
(10.65%). The three examined environments showed different bacterial assemblages; in particular, we observed the enrichment
of members of Bacteroidetes within fecal samples of CRC patients, while Firmicutes were over-represented in the fecal samples
of healthy controls. The CT samples show the highest alpha diversity values.
F. nucleatum
in patients was shown to be more
abundant in saliva samples than in feces samples and, notably related to the presence of metastases. These results highlight
a different taxonomic composition of feces from CRC compared to healthy controls and that the
F. nucleatum
presence is
positively associated with the clinical course of CRC patients (metastasis). So, our results could be useful to promote the
development of novel bacteria-related diagnostic tools and therapeutic interventions in CRC patients.
edda.russo@unifi.itJ Gastrointest Dig Syst 2017, 7:6(Suppl)
DOI: 10.4172/2161-069X-C1-059