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Journal of Chromatography & Separation Techniques | Volume: 09
8th World Congress on Chromatography
September 13-14, 2018 | Prague, Czech Republic
Polymer Science and Technology
4 th International Conference on
Joint Event on
&
Joanna Fiedor
AGH-University of Science and
Technology, Poland
Purple non-sulphur phototrophic bacteria constitute a unique group of
“photosynthetic” organisms capable of adjusting their metabolism in response
to the alteration of environmental growth conditions. In the presence of light and
absence of oxygen, bacterial cells develop extensive system of intracytoplasmic
membranes, hosting entire “photosynthetic machinery”. It comprises different
types of pigment-protein complexes involved in capturing and extremely effective
conversion of light energy into chemical energy. Recently, purple bacteria have
been gaining considerable attention due to their increasing potential in a range
of scientific and industrial applications. In the present study two species of
anoxygenically grown phototrophic bacteria were used. Following the isolation of
their membranes, the components of the photosynthetic apparatus were separated
and purified by the application of weak anion exchange chromatography. This
chromatographic technique is characterized by relative simplicity, vast availability
as well as high effectivenes of structure’s separation. Hence, it proved to be one
of the most useful methods for isolation and purification of membrane as well as
water-soluble proteins. Application of ion-chromatography resulted in preparation
of a series of photosynthetic (pigment-) protein structures of adequate purity for
Joanna Fiedor, J Chromatogr Sep Tech 2018, Volume: 09
DOI: 10.4172/2157-7064-C2-042
Chromatographic purification of photosynthetic water-soluble and membrane proteins for
spectrometric analysis
spectrometric analysis. Here, total reflection X-ray fluorescence spectrometry (TXRF), one of the well-established spectroscopic
techniques applied for the precise elemental profiling of organic and inorganic samples, was used to perform comprehensive
examination of the elements present in bacterial proteins.
Biography
Joanna Fiedor has received her PhD degree in Biochemistry from the Jagiellonian University, Kraków, Poland. During 1997-1999, she visited the Ludwig-
Maximilians University (LMU) in Munich, Germany, and in 2002 the Kwansei Gakuin University, Sanda, Japan. Currently, she is an Assistant Professor at the AGH-
University of Science and Technology, Kraków, Poland. Her research interests are focused on: natural biocompounds (carotenoids) in relation to human health, and
biotechnological applications of phototrophic microorganisms.