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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
J Chromatogr Sep Tech 2016, 7:5(Suppl)
Isolation of ulceroprotective cucurbitane type triterpenoids from Cucumis melo seeds
Gurpreet Singh Bal and Naresh Singh Gill
Punjab Technical University, India
Medicinal plants are the richest bio-resources of drugs in traditional medicinal systems, modern medicines, folk
medicines, intermediate and chemicals entitled for synthetic drugs. Plants provide a source of inspiration for novel drug
development as they contain a vast array of substances that treat chronic diseases. Cucumis melo seeds have been traditionally
used for treating various health ailments. The main aim of our current study is to isolate cucurbitane-type triterpenoids from
Cucumis melo seed extract and conduct anti-ulcerogenic activity of the isolated compound. Phytochemical investigations of
methanolic seed extract of Cucumis melo was carried out which showed the presence of various important phytoconstituents.
The main active constituents of Cucumis melo have shown a number of potent pharmacological activities. The isolation of
Cucurbitane-type triterpenoids was carried out by column chromatography using methanolic seed extract of Cucumis melo.
Mobile phase hexane and hexane-ethyl acetate (98:2) was used to run the column. TLC profiling was done simultaneously in
an appropriate solvent system (hexane: ethyl acetate, 97:3). Various fractions were collected. The fractions with similar Rf value
were pooled together. Fractions giving single spot in the TLC were regarded as pure. The isolated compound showed positive
result for Liebermann-Burchard test from which we can conclude that the isolated compound might be triterpenoid. The
structure of the isolated compound was determined by IR, 1HNMR, 13CNMR techniques. The spectral analysis of the isolated
compound showed following results: IR- it showed the peaks at 3383, 2976, 2814, 1721, 1465, 1123 cm-1 indicated the presence
of alcoholic group.
Results on the determination of fatty acids in biological samples by applying gas chromatography
Ropota Mariana and Olteanu Margareta
IBNA Balotesti, Romania
Analytical method validation is the confirmation by examination and provision of objective evidence that certain specific
requirements for intentional application are achieved. So validation of analytical quality assurance represents the first
step in a laboratory. The fatty acids were determined by gas chromatography which involves the transformation of the fatty
acids from the sample in methyl esters and separation of the components in the chromatographic column, their identification
by comparison with the standard chromatograms. The method complies with standard SR CEN ISO/TS 17764 -2: 2008,
used a Perkin Elmer-Clarus 500 chromatograph with capillary injection, high polarity stationary phase (BPX70: 60 mx0.25
mm inner diameter and 0.25 µm film thickness). The method was validated “in house”, and used as methylated fatty acids
standard solution Mix 37 Component FAME; 10 mg/mL, (CRM) soybean oil. We determined the following parameters:
accuracy=98.72%, coefficient of variation of repeatability RSD=0.414%, detection limit LoD=0.002349 µg/mL, quantification
limit LoQ=0.05683 µg/mL and recovery R=98.84%, according to SR EN ISO/CEI 17025: 2005, all values being within the
admitted range: RSD: 80–120%, LoQ>LoD and 80<R>120%. The following concentrations of fatty acids were determined in
samples of eggs, expressed per 100 g fat extracted from the yolk. Thus, α-linolenic acid has ranged between: 0.22±0.3 g (C)
and 1.19±0.13 g/100 g fat, total omega-3 has values between: 1.37±0.09 g (C) 4.84±0.32 g (E1) and total omega-6 has values
between: 24.61±1.38 g (C) and 20.91±1.08 g/100 g fat (E1).