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Volume 9
Journal of Obesity & Weight Loss Therapy
ISSN: 2165-7904
JOINT EVENT
Childhood Obesity 2019
Diabetes Conference 2019
March 18-19, 2019
&
3
rd
World Congress on
Diabetes and Obesity
12
th
International Conferences on
Childhood Obesity and Nutrition
March 18-19, 2019 | Rome, Italy
Glucagon-like peptide-1 promotes α to β cell Trans-differentiation in the absence of MafA
Chuan Zhang
Second Hospital of Jilin University, China
Statement of the Problem:
Diabetes is a chronic disease caused by the relative or absolute lack of functional
pancreatic β cells and is characterized by chronic hyperglycemia. Type 2 diabetes islets exhibit worse glucose-
stimulated insulin secretion than normal islets and a decrease in MafA expression. Researchers have reported that
GLP-1 plays important roles in the differentiation and regeneration of pancreatic β cells. However, this perspective
that GLP-1 can play an important role in α cell trans-differentiation under the condition of MafA gene deletion and
can produce new insulin-producing cells has not been previously studied. The purpose of this study is that glucagon-
like peptide-1 promotes α to βtrans-differentiation in the absence of MafA.
Methodology & Theoretical Orientation:
Male MafA-deficient mice were injected GLP-1 (50 µg/kg body weigh)
daily for 4 weeks. Blood glucose level and body weight were measured, and the numbers of immunofluorescent islet
α cells and β cells were analyzed by Immunohistochemical analysis. Real-time quantitative PCR was used to analysis
the change in related transcription.
Findings:
GLP-1-treated mice exhibit improved blood glucose levels without hypoglycemia. Mice injected with
GLP-1 increase β cell regeneration by promoting α-β cell trans-differentiation. GLP-1-treated mice may induce the
conversion of α cells into β cells by inducing PDX-1 production.
Conclusion & Significance:
GLP-1 can reduce fasting blood glucose, decrease body weight, increase β cell
regeneration by promoting α to β cell trans-differentiation. This research provides an new incisive point for the
treatment of diabetes mellitus and provide the theoretical and experiment bases for treatment of diabetic β cell
failure.
Biography
Chuan Zhang has her expertise in evaluation and passion in improving the diabetes and Obesity. She has been engaged in clinical and scientific research for
more than 30 years, and she tries to combine the knowledge and technology she has learned in Japan with his clinical work. She has designed this experiment
after years of experience in research, evaluation, teaching and administration both in hospital and education institutions. This research provides an new incisivus
point for the treatment of diabetes mellitus and provide the theoretical and experiment bases for treatment of diabetic β cell failure.
wangs93@sina.comChuan Zhang, J Obes Weight Loss Ther 2019, Volume 9
DOI: 10.4172/2165-7904-C1-091