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conferenceseries.com
Volume 7, Issue 3 (Suppl) J Obes Weight Loss Ther, an open access journal
ISSN: 2165-7904 Childhood Obesity & Bariatric Surgery 2017
June 12-13, 2017
June 12-13, 2017 Rome, Italy
& Childhood Obesity and Nutrition
10th International Conference on
Metabolic and Bariatric Surgery
2nd International Conference on
JOINT EVENT
No association between LEPR Q223R and plasma leptin level in Caucasian female adolescents
Kseniia Dmitrievna Ievleva, Ekaterina A Sheneman, Tatyana A Bairova and Lubov V Rychkova
SC FHHRP-Siberian Branch of the Russian Academy of Sciences, Russia
Statement of the Problem: The hormone leptin are involved in regulation of energy metabolism. Effect of the leptin carried out
through binding to its receptor in target tissues. Many polymorphisms of LEPR gene were found out including Q223R (Gln223Arg).
This polymorphism produces molecular changes in extracellular region of the LEPR. Several studies showed the association of
polymorphism with obesity and leptin level in adult and adolescent samples. However, some studies showed no association.
Methodology &Theoretical Orientation: A total of 128 Caucasian female adolescents living in Eastern Siberia (Irkutsk city, Russia)
were observed. 59 of them were included in control group (average age 15.72±0.93; SDS BMI 0.84±0.55) and 69 adolescents with
overweight and obesity was in main group (average age 16.04±1.07; SDS BMI 2.52±0.72). Plasma leptin level (PLL) was determined
using commercially available enzyme-linked immunosorbent assay (LEPTIN ELISA kit, DBC, Canada) with an absorbance micro-
plate reader MultiSkan ELX808 (Biotek, USA). Genomic DNA was extracted from EDTA-treated whole blood by commercial
kits (DNA-Sorb-B, AmpliSens, Russia). Genotyping LEPR Q223R was performed using polymerase chain reaction (Eppendorf
Mastercycler Gradient PC, Germany) with electrophoresis detection. Statistical analysis was performed by soft “STATISTICA8.0”.
Findings: The plasma leptin level was significantly higher inmain group (50.57±16.06) than in control group (23.91±14.13) (p<0.001).
G-allele frequency was 43.1% in control and 40% in main group (р=0.862). Comparisons of the meaning circulating leptin levels
stratified by LEPR Q223R genotype showed in table 1. We didn’t find significant differences of leptin level between carriers of AA,
AG and GG genotypes in both groups.
Conclusion & Significance: Thus, The plasma leptin level is increased in female adolescent with overweight and obesity. There is no
significant association of LEPR Q223R with plasma leptin level and obesity in Caucasian female adolescents living in Eastern Siberia.
Biography
Kseniia Dmitrievna Ievleva finished the Faculty of Medical Biochemistry, Irkutsk State Medical University in 2014. In 2015, she got the certificate of Doctor of
Laboratory. Now, she is a Post-graduate student in Scientific Centre for Family Health Problems and Human Reproduction. Her present study aims to “Examine the
role of heredity in the development of obesity in adolescents living in Eastern Siberia”.
Kseniia Dmitrievna Ievleva et al., J Obes Weight Loss Ther 2017, 7:3 (Suppl)
DOI: 10.4172/2165-7904-C1-046