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Volume 9
Journal of Chemical Engineering & Process Technology
Catalysis 2018
September 05-06, 2018
September 05-06, 2018 Tokyo, Japan
5
th
World Congress on
Catalysis and Chemical Engineering
Carlos Zambra et al., J Chem Eng Process Technol 2018, Volume 9
DOI: 10.4172/2157-7048-C2-015
Increases of the polyphenols concentration in the aqueous phase of the olive oil wastes by biocatalysis
Carlos Zambra
1
, Susana Nieto Cerón
2
and Pedro Lozano
2
1
University of Talca, Chile
2
University of Murcia, Spain
T
he intake of natural antioxidants and waste valorization are issues of great importance for improving human health and
achieve a modern and efficient industry. Powerful natural antioxidants can be found in high concentrations in liquid waste
from the olive oil industry. In this work, the effect of enzymes to increase the polyphenol concentration of the aqueous phase
fromolive oil wastes (alperujo) was studied. The high concentration of fiber in this waste suggest that biocatalysts with cellulases
would useful for obtain an increase of the polyphenols concentration. Two commercial enzymes were used in different rate and
concentrations. The total polyphenol content and the presence of free carbonyl group (reducing sugars) was determined by
colorimetric methods with the Folin-Ciocalteu and the 3,5-Dinitrosalicylic acid (DNS) reagent, respectively. A liquid-liquid
process enzymatic assisted was carry out by one hour at 50 °C and increased the phenolic content up to 1000% compared with
the same test without enzymes. Use enzymes improve significantly the polyphenol concentration of the aqueous phase in olive
oil wastes. The enzymes can be used to catalyze polyphenols extraction process from the olive oil wastes and thus to make the
process more efficiently and economically sustainable.
Biography
Carlos Zambra has his expertise developing mathematical models and numerical simulations. His main interests are in the problems that involve chemical and
biochemical reactions and new process such as liquid-liquid extractions, membrane perstraction, pervaporation and membrane distillation.
carlos.zambra.s@gmail.com