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Notes:
Volume 9, Issue 5 (Suppl) J Cancer Sci Ther, an open access journal
ISSN: 1948-5956 Cancer Stem Cells and Oncology Research 2017
June 26-28, 2017
Page 57
10th International Conference on
June 26-28, 2017 London, UK
CANCER STEM CELLS AND
ONCOLOGY RESEARCH
Cell-based therapy using miR-302-367 expressing cells represses glioblastoma development
Thierry Virolle
Institut de Biologie Valrose- Ibv, France
Glioblastomas are incurable primary brain tumors that affect patients of all ages. The aggressiveness of this cancer has been
attributed in part to the persistence of treatment-resistant glioblastoma stem-like cells (GSC). We have demonstrated that
microRNA cluster miR-302-367 has the potential to force GSC exit from stemness, promoting loss of stemness properties and
tumorigenicity and revealing a great therapeutic interest. In our study we attempt to develop a cell-based therapy for miR-302-367
continuous delivery by taking advantage of the capability of glioma cells to secrete exosomes that enclose small RNA molecules.
We engineered primary glioma cells to stably express the miR-302-367. Remarkably, these cells altered, in a paracrine manner, the
expression of stemness markers, the proliferation and the tumorigenicity of neighboring glioblastoma cells. Further characterization
of the secretome derived from miR-302-367 expressing cells showed that a large amount of miR-302-367 was enclosed in exosomes,
which were internalized by the neighboring glioblastoma cells. This miR-302-367 cell-to-cell transfer resulted in the inhibition of its
targets such as CXCR4/SDF1, SHH, cyclin D, cyclin A and E2F1. Orthotopic xenograft of miR-302-367-expressing cells together with
glioblastoma stem-like cells efficiently altered initiation and tumor development in mice brain.
Biography
Thierry Virolle is a Research Director (permanent position) at Institut National de la Santé et de la Recherche Médicale (INSERM), Head of the Team Cancer Stem
Cell Plasticity and Functional intra-tumor Heterogeneity at the Institute of Biologie Valrose (iBV). He is Co-Founder of the French National Sud Cancer Stem Cell
Network, SUNRiSE dedicated to the study of cancer stem cell. He is Doctor of Science (PhD) at Nice Sophia Antipolis University (2000), his researches focus on
the regulation of the plasticity of glioblastoma cancer stem cells and its contribution in the genesis of functionally divergent tumor territories.
Thierry Virolle, J Cancer Sci Ther 2017, 9:5(Suppl)
DOI: 10.4172/1948-5956-C1-102