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Volume 3
Journal of Cancer Diagnosis
Cancer Diagnostics Conference 2018
August 15-16, 2018
Page 13
Notes:
conference
series
.com
August 15-16, 2018 Singapore
International Conference on Cancer Research & Diagnostics
&
16
th
Asia Pacific Biotechnology Congress
Joint Event on
Uncover the molecular mechanism that regulates the RAF/MEK/ERK kinase cascade by characterizing the
oncogenic RAF/MEK mutants
A
lthough extensively studied for three decades, the molecular mechanism that regulates the RAF/MEK/ERK cascade
remains ambiguous. Recent studies identified the dimerization of RAF as a key event in the activation of this cascade. Here,
we show that in-frame deletions in the β3-αC loop activate ARAF, BRAF and MEK1 as well as other kinases in cancer genomes
by enforcing homodimerization. These RAF and MEK1 mutants exhibit a strong oncogenic potential and differential inhibitor
resistance. Using these unique mutants, we further demonstrate that RAF activates MEK in a dimer-to-dimer manner and
that MEK is able to activate itself by homodimerization-driven transphosphorylation. This study defines a special catalogue of
oncogenic kinase mutations and illustrates key steps in the activation of the RAF/MEK/ERK cascade.
Biography
Jiancheng Hu has completed his PhD training in 2007 from University of Colorado Health Sciences Centre and Postdoctoral studies in 2014 from Howard Hughes
Medical Institute and Washington University in St. Louis. He is the Principal Investigator of Cellular and Molecular Research Division, National Cancer Centre
Singapore. He has published more than 15 papers in reputed journals.
hu.jiancheng@nccs.com.sgJiancheng Hu
National Cancer Centre Singapore, Singapore
Jiancheng Hu, J Cancer Diagn 2018, Volume 3
DOI: 10.4172/2476-2253-C1-001