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conferenceseries
.com
Volume 9
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
JOINT EVENT
February 28-March 02, 2019 | Berlin, Germany
5
th
International Conference on
Enzymology and Protein Chemistry
&
22
nd
Global Congress on
Biotechnology
Biotechnology 2019
Enzymology 2019
February 28-March 02, 2019
&
Treatment of gamma radiation damage in male rats by using of stem cells and silymarin
Ashraf Z M
Mansoura University, Egypt
T
herapeutic effect of mesenchymal stem cells transplantation (MSCs) and an antioxidant such as silymarin have been
postulated as hepatoprotectors against ionizing radiation induced injury. The present study was undertaken to evaluate the
protective effect of MSCs and silymarin to ameliorate damage caused by gamma radiation. Bratton-Marshall Reagent was given
by intravenous injection to male rats, one day post gamma irradiation at the dose level of 4 Gy. Rats were orally administrated
silymarin at dose (70 mg/kg dissolved in distilled water) before irradiated three days and continued for 21 days post irradiation.
After one and three weeks post irradiation results revealed that irradiated animals receiving MSCs and silymarin separately or
with each other exhibited a pronounced elevation in liver antioxidant such as glutathione (GSH) superoxide dismutase (SOD),
glutathione-S-transferase (GST), total antioxidant capacity (TAC), catalase (CAT) and glucose-6-phosphate dehydrogenase
(G-6-PDase) activity accompanied with significant decline in lipid peroxidation and hydrogen peroxide levels in comparing
with irradiated rats. Moreover, RAPD-PCR with primers OP-B10 and OP-B14 exhibited different banding patterns in all
treated rats compared to untreated control rats after one and three weeks of treatment. In conclusion, treatment with MSCs
and silymarin possess a radio protective capacity against ionizing-radiation induced oxidative stress and organ injury.
ashrafzakaria7878@yahoo.comJ Biotechnol Biomater 2019, Volume 9
DOI: 10.4172/2155-952X-C2-116