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Page 58

Bioplastics 2016

November 10-11, 2016

Volume 7 Issue 6(Suppl)

J Bioremediat Biodegrad

ISSN: 2155-6199 JBRBD, an open access journal

conferenceseries

.com

November 10-11, 2016 Alicante, Spain

International Conference on

Sustainable Bioplastics

Veronica Carbonell, J Bioremediat Biodegrad 2016, 7:6(Suppl)

http://dx.doi.org/10.4172/2155-6199.C1.006

Development of stable recombinant

cyanobacteria

for economically competent solar-fuel-factories:

ethylene production

Veronica Carbonell

University of Turku, Finland

G

reenhouse gas emissions and limited fossil fuel reserves increase the need to find alternative ways to generate substitutes

for petroleum-derived products such as ethylene. Ethylene is a simple alkene of commercial value due to multitude of

large-scale uses in plastic industry and ever growing demand. One of the promising approaches is to use

cyanobacterial

cells as

biological factories, through their photosynthetic capacity to produce ethylene using atmospheric CO

2

and water as substrates.

The biosynthesis of ethylene has been studied in

Synechococcus

sp 7942 by over-expressing the heterologous ethylene forming

enzyme (efe) from

Pseudomonas syringae

which converts the endogenous metabolic precursor 2-oxoglutarate to ethylene. As

a volatile gas, ethylene then diffuses out from the cell and spontaneously separates into the culture headspace for collection

and analysis. We have studied different aspects of observed genetic instability which have earlier compromised prolonged

ethylene production in

Synechococcus

, and have developed stable production strains capable of sustained autotrophic

ethylene biosynthesis. Although the production levels still remain below the threshold required for commercial applications,

cyanobacteria have been intensively studied in this respect, and a range of molecular biology tools and production platforms

are being developed and characterized.

Biography

Veronica Carbonell is Licentiate on Environmental Sciences by the University of Miguel Hernandez with strong international background and has participated

in international programs such as Erasmus at Free University of Brussels (Belgium), FARO at John Innes Center (UK) and IAESTE at Silesian University of

Technology (Poland). Currently, she is pursuing her PhD from the University of Turku, Finland.

vecago@utu.fi