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Volume 8 Journal of Biotechnology and Biomaterials
ISSN: 2155-952X Biomaterials 2018
March 05-06, 2018
March 05-06, 2018 | Berlin, Germany
3rd Annual Conference and Expo on
Biomaterials
Extended spectrum beta-lactamase in clinical isolates of Escherichia coli and Klebsiella pneumoniae
from the Tamale teaching hospital
F K M Tetteh1, J A Opintan1, A Ablordey 2 and N Obeng Nkrumah1
1University of Ghana, Ghana
2Noguchi Memorial Institute for Medical Research, Ghana
Extended spectrum beta-lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae are pathogens of
significant public health interest to which new antibiotics therapies are urgently needed. This study was designed to
determine the prevalence of ESBLs in clinical isolates of E. coli and K. pneumoniae from patients attending the Tamale
teaching hospital (TTH). A total of 140 isolates of E. coli (83.6%; n=117) and K. pneumoniae (16.4%; n=23) were cultured from
clinical specimens of consenting patients. Antimicrobial susceptibility was determined using the Kirby-Bauer disc diffusion
method. Screening and confirmation for ESBL-producing phenotypes among the clinical isolates were performed according
to the guidelines of the Clinical and Laboratory Standard Institute, 2012. Escherichia coli and K. pneumoniae positive for
ESBL phenotype were examined for the presence of TEM, SHV and CTX-M genes. Sixty two (44.3%) of the 140 isolates
expressed ESBLs phenotypically. Of these, 83.9% (n=52) were E. coli and 16.1% (n=10) were K. pneumoniae isolates. The
proportion of ESBL-producing isolates were found to be relatively higher in adults (15-65 years) than in neonates (<28 days)
[p=0.14]. Majority of the isolates showed high percentage resistance to ampicillin (96%) and tetracycline (89%), but relatively
low resistance for amikacin (36%). None of the isolates were resistant to meropenem. The ESBL producers were multidrug
resistant compared to non-ESBL-producers (23%, n=14/62 versus 18%, n=14/78; p=0.573). Overall, 74.2% (n=46/62) of the
ESBL genotypes expressed BlaCTX-M-1 genes followed by 62.9% (n=39/62) BlaTEM and 16.1% (n=10/62) BlaSHV. Two
(3.2%) isolates had both TEM and SHV genes, 29 (46.8%) harbored TEM and CTX-M-1, 2 (3.2%) had SHV and CTXM-1,
while 4 (6.5%) harbored all three genes. None expressed genes for CTX-M 2 and CTX-M 9. In univariate comparisons, patients
who reported their previous medication as having being prescribed by a Physician and those who reportedly completed their
previous medication were more likely to be infected by ESBL organisms. The study showed high ESBL positive E. coli and K.
pneumoniae, mostly CTX-M-1 producers in Tamale teaching hospital. Routine laboratory ESBL detection is warranted.
J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C1-089