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.com
Volume 8, Issue 5 (Suppl)
J Bioremediat Biodegrad, an open access journal
ISSN: 2155-6199
Biofuels Congress 2017
September 05-06, 2017
September 05-06, 2017 | London, UK
Biofuels and Bioenergy
6
th
World Congress on
The reconstruction of metabolic pathways in selected bacterial and yeast strains for production of
bioethylene from crude glycerol
Chiyanzu I
and
Mangena M
ARC-IAE, RSA
C
rude glycerol, a major by-product from the transesterification of Sunflower oil with alcohol to biodiesel, can pose danger
to the environment in large quantities. Studies have shown that utilization of the glycerol to afford commercial products is
one of the promising options for lowering its pollution effects and biodiesel production costs. For example, its bioconversion
can offer a wide range of chemicals including alcohols, organic acids, hydrogen, solvents and precursors for bioplastics. In
Pseudomonas
syringae
species of bacteria, the 2-oxoglutarate dioxygenase (2-OGD) are widely known to be among the enzymes
with an emerging importance in ethylene formation. However, the optimization and industrial applications of enzyme as
recombinant systems for crude glycerol conversion to ethylene is still not been reported. The present study investigated the
production of ethylene from crude glycerol using engineered
Pichia pastoris, E. coli
MG1655 and JM109 strains. Ethylene
production with a codon-optimized expression system for 2-OGD in
E.coli
using a codon optimized construct of the ethylene-
forming gene was studied. The effect of codon optimization resulted in a 20-fold increase of protein production and thus an
enhanced production of the ethylene gas. For a reliable bioreactor performance, the effect of temperature, fermentation time,
pH, substrate concentration, concentration of methanol, concentration of potassium hydroxide and media supplements on
ethylene yield was investigated. The results demonstrate that the recombinant enzyme can be used for future studies to exploit
the conversion of low-priced crude glycerol into advanced value products like light olefins, and tools including recombineering
techniques for DNA, molecular biology and bioengineering can be used to allowing unlimited the production of ethylene
directly from fermentation of crude glycerol. It can be concluded that recombinant E.coli production systems represent
significantly secure, renewable and environmentally safe alternative to thermochemical approach to ethyleneproduction.
ChiyanzuI@arc.agric.zaJ Bioremediat Biodegrad 2017, 8:5(Suppl)
DOI: 10.4172/2155-6199-C1-009